LOCUS       sequence-153603-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: scTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP277
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(587..833)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(875..901)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctcaccatga attcttttgt tgttgagtga agcgagtgac ggaacggtaa aatgtaagta
       61 acaaaagaaa aagagaacca ggggggggag gagagtatgt atttataccg tacggcacca
      121 ggcgaaaagc tataaacaaa cctttttcgc ggtatatttg tttatatttc ctattttaaa
      181 ctcaaaatct gccctaggcg cgcccatagg gtatcctcac tccgattgat gaagagaccc
      241 ttctggttcc aatcctcact ccgattgatg tgacgtcaat gggtggagta tatcctcact
      301 ccgattgatg agtgctctcg atgacttcga tatcctcact ccgattgatg aactccgccc
      361 cattgacgca aatcctcact ccgattgatg gtggaacagc ccaccctgta catcctcact
      421 ccgattgatg tgcggcctcg agaagcagct gaatcctcac tccgattgat gactggaccg
      481 tcaccaattg gagatcctca ctccgattga tggtggtaaa ctcgcaatat cctcactccg
      541 attgatgaat tgatctgccg cgatgacttc tatatgatat gctagctctc acttaatctt
      601 ctgtactctg aagaggagtg ggaaatacca agaaaaacat caaactcgaa tgattttccc
      661 aaacccctac cacaagatat tcatcagctg cgagataggc tgatcaggag caagctcgta
      721 cgagaagaaa caaaatgaca aaaaaaatcc tatactatat aggttacaaa taaaaaagta
      781 tcaaaaatga agcctgcatc tctcaggcaa atggcattct gacatcctct tgattagaat
      841 ctagcaagac cggtctaccg atggtacctc aagaagcgta gtccggaacg tcgtacgggt
      901 agttaattaa catatcgaga tcga
//
