LOCUS       sequence-153607-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: ppTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP190
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(582..828)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(870..896)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 catgaattcg cgtttcgaat aattagttgt tttttgatct tctcaagttg tcgttaaaag
       61 tcgttaaaat caaaagcttg tcaattggaa ccagtcgcaa ttatgaaagt aagctaataa
      121 tgatgataaa aaaaaaggtt taagacaggg cagcttcctt ctgtttatat attgctgtca
      181 agtaggggtt aggcgcgccc atagggtatc ctcactccga ttgatgaaga gacccttctg
      241 gttccaatcc tcactccgat tgatgtgacg tcaatgggtg gagtatatcc tcactccgat
      301 tgatgagtgc tctcgatgac ttcgatatcc tcactccgat tgatgaactc cgccccattg
      361 acgcaaatcc tcactccgat tgatggtgga acagcccacc ctgtacatcc tcactccgat
      421 tgatgtgcgg cctcgagaag cagctgaatc ctcactccga ttgatgactg gaccgtcacc
      481 aattggagat cctcactccg attgatggtg gtaaactcgc aatatcctca ctccgattga
      541 tgaattgatc tgccgcgatg acttctatat gatatgctag ctctcactta atcttctgta
      601 ctctgaagag gagtgggaaa taccaagaaa aacatcaaac tcgaatgatt ttcccaaacc
      661 cctaccacaa gatattcatc agctgcgaga taggctgatc aggagcaagc tcgtacgaga
      721 agaaacaaaa tgacaaaaaa aatcctatac tatataggtt acaaataaaa aagtatcaaa
      781 aatgaagcct gcatctctca ggcaaatggc attctgacat cctcttgatt agaatctagc
      841 aagaccggtc taccgatggt acctcaagaa gcgtagtccg gaacgtcgta cgggtagtta
      901 attaacatat cgagatcga
//
