LOCUS       sequence-153614-       10195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insulin receptor isoform A with a inter-domain EBFP2 tag at 
            extracellular region (between furin-like domain and transmembrane 
            domain). For imaging insulin receptor trafficing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P,
            Johnson JD
  TITLE     Inter-domain tagging implicates caveolin-1 in insulin receptor 
            trafficking and Erk signaling bias in pancreatic beta-cells.
  JOURNAL   Mol Metab. 2016 Feb 10;5(5):366-78. doi: 
            10.1016/j.molmet.2016.01.009. eCollection 2016 May.
  PUBMED    27110488
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molmet.2016.01.009"; journalName: "Mol Metab"; date: 
            "2016-02-10- 10"; volume: "5"; issue: "5"; pages: "366-78"
COMMENT     Sequence Label: InsRA-EBFP2
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      915..924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3741..3743,3744..3746,3747..4457)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(3786..3807)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5789..5806)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5795..6019
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6065..6493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6152..6171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6362..6383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6502..6522)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6507..6836
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6687..6822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6903..7697
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6957..6976)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7567..7586
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7871..7992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7908..7927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7962..7981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8041..8057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8041..8057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8054..8076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8065..8081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8089..8095,8096..8113,8114..8119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8134..8155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8272..8289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8443..9028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8523..8542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9199..9990,9991..10059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9822..9841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10060..10164)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gcccgccgcc atgggcaccg ggggccggcg gggggcggcg gccgcgccgc
      961 tgctggtggc ggtggccgcg ctgctactgg gcgccgcggg ccacctgtac cccggagagg
     1021 tgtgtcccgg catggatatc cggaacaacc tcactaggtt gcatgagctg gagaattgct
     1081 ctgtcatcga aggacacttg cagatactct tgatgttcaa aacgaggccc gaagatttcc
     1141 gagacctcag tttccccaaa ctcatcatga tcactgatta cttgctgctc ttccgggtct
     1201 atgggctcga gagcctgaag gacctgttcc ccaacctcac ggtcatccgg ggatcacgac
     1261 tgttctttaa ctacgcgctg gtcatcttcg agatggttca cctcaaggaa ctcggcctct
     1321 acaacctgat gaacatcacc cggggttctg tccgcatcga gaagaacaat gagctctgtt
     1381 acttggccac tatcgactgg tcccgtatcc tggattccgt ggaggataat cacatcgtgt
     1441 tgaacaaaga tgacaacgag gagtgtggag acatctgtcc gggtaccgcg aagggcaaga
     1501 ccaactgccc cgccaccgtc atcaacgggc agtttgtcga acgatgttgg actcatagtc
     1561 actgccagaa agtttgcccg accatctgta agtcacacgg ctgcaccgcc gaaggcctct
     1621 gttgccacag cgagtgcctg ggcaactgtt ctcagcccga cgaccccacc aagtgcgtgg
     1681 cctgccgcaa cttctacctg gacggcaggt gtgtggagac ctgcccgccc ccgtactacc
     1741 acttccagga ctggcgctgt gtgaacttca gcttctgcca ggacctgcac cacaaatgca
     1801 agaactcgcg gaggcagggc tgccaccaat acgtcattca caacaacaag tgcatccctg
     1861 agtgtccctc cgggtacacg atgaattcca gcaacttgct gtgcacccca tgcctgggtc
     1921 cctgtcccaa ggtgtgccac ctcctagaag gcgagaagac catcgactcg gtgacgtctg
     1981 cccaggagct ccgaggatgc accgtcatca acgggagtct gatcatcaac attcgaggag
     2041 gcaacaatct ggcagctgag ctagaagcca acctcggcct cattgaagaa atttcagggt
     2101 atctaaaaat ccgccgatcc tacgctctgg tgtcactttc cttcttccgg aagttacgtc
     2161 tgattcgagg agagaccttg gaaattggga actactcctt ctatgccttg gacaaccaga
     2221 acctaaggca gctctgggac tggagcaaac acaacctcac caccactcag gggaaactct
     2281 tcttccacta taaccccaaa ctctgcttgt cagaaatcca caagatggaa gaagtttcag
     2341 gaaccaaggg gcgccaggag agaaacgaca ttgccctgaa gaccaatggg gacaaggcat
     2401 cctgtgaaaa tgagttactt aaattttctt acattcggac atcttttgac aagatcttgc
     2461 tgagatggga gccgtactgg ccccccgact tccgagacct cttggggttc atgctgttct
     2521 acaaagaggc cccttatcag aatgtgacgg agttcgatgg gcaggatgcg tgtggttcca
     2581 acagttggac ggtggtagac attgacccac ccctgaggtc caacgacccc aaatcacaga
     2641 accacccagg gtggctgatg cggggtctca agccctggac ccagtatgcc atctttgtga
     2701 agaccctggt caccttttcg gatgaacgcc ggacctatgg ggccaagagt gacatcattt
     2761 atgtccagac agatgccacc aacccctctg tgcccctgga tccaatctca gtgtctaact
     2821 catcatccca gattattctg aagtggaaac caccctccga ccccaatggc aacatcaccc
     2881 actacctggt tttctgggag aggcaggcgg aagacagtga gctgttcgag ctggattatt
     2941 gcctcaaagg gctgaagctg ccctcgagga cctggtctcc accattcgag tctgaagatt
     3001 ctcagaagca caaccagagt gagtatgagg attcggccgg cgaatgctgc tcctgtccaa
     3061 agacagactc tcagatcctg aaggagctgg aggagtcctc gtttaggaag acgtttgagg
     3121 attacctgca caacgtggtt ttcgtcccca ggccatctcg gaaacgcagg tcccttggcg
     3181 atgttgggaa tgtgacggtg gccgtgccca cggtggcagc tttccccaac acttcctcga
     3241 ccagcgtgcc cacgagtccg gaggagcaca ggccttttga gaaggtggtg aacaaggagt
     3301 cgctggtcat ctccggcttg cgacacttca cgggctatcg catcgagctg caggcttgca
     3361 accaggacac ccctgaggaa cggtgcagtg tggcagccta cgtcagtgcg aggaccatgc
     3421 ctgaagccaa ggctgatgac attgttggcc ctgtgacgca tgaaatcttt gagaacaacg
     3481 tcgtccactt gatgtggcag gagccgaagg agcccaatgg tctgatcgtg ctgtatgaag
     3541 tgagttatcg gcgatatggt gatgaggagc tgcatctctg cgtctcccgc aagcacttcg
     3601 ctctggaacg gggctgcagg ctgcgtgggc tgtcaccggg gaactacagc gtgcgaatcc
     3661 gggccacctc ccttgcgggc aacggctctt ggacggaacc cacctatttc tacgtgacag
     3721 actatttaga cgtccgtacg atggtgagca agggcgagga gctgttcacc ggggtggtgc
     3781 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg
     3841 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3901 tgcccgtgcc ctggcccacc ctcgtgacca ccctgagcca cggcgtgcag tgcttcgccc
     3961 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4021 tccaggagcg caccatcttc ttcaaggacg acggcaccta caagacccgc gccgaggtga
     4081 agttcgaggg cgacacccta gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg
     4141 acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac atctatatca
     4201 tggccgtcaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacgtggagg
     4261 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4321 tgctgctgcc cgacagccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg
     4381 agaagcgcga tcacatggtc ctgctggagt tccgcaccgc cgccgggatc actctcggca
     4441 tggacgagct gtacaagacc ggtccgtcaa atattgcaaa aattatcatc ggccccctca
     4501 tctttgtctt tctcttcagt gttgtgattg gaagtattta tctattcctg agaaagaggc
     4561 agccagatgg gccgctggga ccgctttacg cttcttcaaa ccctgagtat ctcagtgcca
     4621 gtgatgtgtt tccatgctct gtgtacgtgc cggacgagtg ggaggtgtct cgagagaaga
     4681 tcaccctcct tcgagagctg gggcagggct ccttcggcat ggtgtatgag ggcaatgcca
     4741 gggacatcat caagggtgag gcagagaccc gcgtggcggt gaagacggtc aacgagtcag
     4801 ccagtctccg agagcggatt gagttcctca atgaggcctc ggtcatgaag ggcttcacct
     4861 gccatcacgt ggtgcgcctc ctgggagtgg tgtccaaggg ccagcccacg ctggtggtga
     4921 tggagctgat ggctcacgga gacctgaaga gctacctccg ttctctgcgg ccagaggctg
     4981 agaataatcc tggccgccct ccccctaccc ttcaagagat gattcagatg gcggcagaga
     5041 ttgctgacgg gatggcctac ctgaacgcca agaagtttgt gcatcgggac ctggcagcga
     5101 gaaactgcat ggtcgcccat gattttactg tcaaaattgg agactttgga atgaccagag
     5161 acatctatga aacggattac taccggaaag ggggcaaggg tctgctccct gtacggtgga
     5221 tggcaccgga gtccctgaag gatggggtct tcaccacttc ttctgacatg tggtcctttg
     5281 gcgtggtcct ttgggaaatc accagcttgg cagaacagcc ttaccaaggc ctgtctaatg
     5341 aacaggtgtt gaaatttgtc atggatggag ggtatctgga tcaacccgac aactgtccag
     5401 agagagtcac tgacctcatg cgcatgtgct ggcaattcaa ccccaagatg aggccaacct
     5461 tcctggagat tgtcaacctg ctcaaggacg acctgcaccc cagctttcca gaggtgtcgt
     5521 tcttccacag cgaggagaac aaggctcccg agagtgagga gctggagatg gagtttgagg
     5581 acatggagaa tgtgcccctg gaccgttcct cgcactgtca gagggaggag gcggggggcc
     5641 gggatggagg gtcctcgctg ggtttcaagc ggagctacga ggaacacatc ccttacacac
     5701 acatgaacgg aggcaagaaa aacgggcgga ttctgacctt gcctcggtcc aatccttcct
     5761 aaaagcttaa gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5821 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5881 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5941 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6001 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6061 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6121 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6181 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6241 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6301 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6361 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6421 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6481 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6541 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6601 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6661 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6721 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6781 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6841 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     6901 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6961 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7021 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7081 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7141 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7201 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7261 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7321 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7381 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     7441 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7501 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7561 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7621 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7681 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     7741 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7801 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7861 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7921 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7981 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     8041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8101 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8221 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     9001 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9061 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9121 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9181 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9241 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9301 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9361 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9421 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9481 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9541 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9601 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9661 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9721 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9781 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9841 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9901 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9961 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10021 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10081 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10141 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
