LOCUS       sequence-153615-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human integrin alpha4, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Liu CC, Leclair P, Yap SQ, Lim CJ
  TITLE     The membrane-proximal KXGFFKR motif of alpha-integrin mediates 
            chemoresistance.
  JOURNAL   Mol Cell Biol. 2013 Nov;33(21):4334-45. doi: 10.1128/MCB.00580-13. 
            Epub 2013 Sep 3.
  PUBMED    24001772
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00580-13"; journalName: "Mol Cell Biol"; date: 
            "2013-11"; volume: "33"; issue: "21"; pages: "4334-45"
COMMENT     Sequence Label: pcDNA3.1-alpha4  wt (Hygro)
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(144..473)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      158..293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(212..231)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     458..478
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(487..915)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(597..618)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     809..828
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
       61 tcgacagacg tcgcggtgag ttcaggcttt ttcatcacgt gctgatcaga tccgaaaatg
      121 gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
      181 tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat aaaaaaaatt
      241 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
      301 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
      361 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
      421 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
      481 agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
      541 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
      601 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
      661 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
      721 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
      781 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
      841 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
      901 cgctacaggg cgcgtgggga taccccctag agccccagct ggttctttcc gcctcagaag
      961 ccatagagcc caccgcatcc c
//
