LOCUS       sequence-153625-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express TagRFP labeled human Rab5a (GTPase located on recycling 
            endosomal membranes)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P,
            Johnson JD
  TITLE     Inter-domain tagging implicates caveolin-1 in insulin receptor 
            trafficking and Erk signaling bias in pancreatic beta-cells.
  JOURNAL   Mol Metab. 2016 Feb 10;5(5):366-78. doi: 
            10.1016/j.molmet.2016.01.009. eCollection 2016 May.
  PUBMED    27110488
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molmet.2016.01.009"; journalName: "Mol Metab"; date: 
            "2016-02-10- 10"; volume: "5"; issue: "5"; pages: "366-78"
COMMENT     Sequence Label: pTag-RFP-C-h-Rab4a-c-Myc
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..757
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgtctaaggg
       61 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa
      121 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
      181 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
      241 cttcatgtac ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa
      301 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
      361 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
      421 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
      481 ggccaacacc gagatgctgt accccgctga cggcggcctg gaaggcagaa gcgacatggc
      541 cctgaagctc gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa
      601 gaaacccgct aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag
      661 aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata
      721 ctgcgacctc cctagcaaac tggggcacaa acttaattcc ggactcagat ctcgagctca
      781 aatgtcgcag acggccatgt ccgaaaccta cgattttttg tttaagttct tggttattgg
      841 aa
//
