LOCUS       sequence-153635-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adeno-associated virus plasmid, Cre-dependent FLEX switch, 
            erHRP(N175S mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Joesch M, Mankus D, Yamagata M, Shahbazi A, Shalek R, Suissa-Peleg 
            A, Meister M, Lichtman JW, Scheirer WJ, Sanes JR
  TITLE     Reconstruction of genetically identified neurons imaged by 
            serial-section electron microscopy.
  JOURNAL   Elife. 2016 Jul 7;5. pii: e15015. doi: 10.7554/eLife.15015.
  PUBMED    27383271
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-07-7"; volume: "5. pii: e15015. doi"; pages: " 
            10.7554/eLife.15015"
COMMENT     Sequence Label: AAV-CAG-DIO-erHRP(N175S mutant)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(131..164)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     178..237
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             241..267
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag
       61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg
      121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc cgccaccatg
      181 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      241 tatccatatg atgttccaga ttatgctatg cagcttactc caactttcta cgataactct
      301 tgtcctaacg tgagcaacat cgtgcgagat actatcgtga acgagttgcg gtctgatccg
      361 aggatagccg cttcaatttt gcggctgcat ttccacgatt gttttgtaaa cggctgtgat
      421 gcttccatcc tcctcgacaa taccacctct ttcaggactg aaaaggatgc cttcggtaac
      481 gcgaatagtg ctagagggtt tcccgtgatc gatagaatga aagccgctgt ggagagcgcc
      541 tgcccccgga cagttagttg tgcagacctt ctgaccatag cggcccagca atccgtgacg
      601 ctggccggtg gccccagctg gagggtcccc ctgggaagga gggactctct gcaggctttt
      661 ctggacttgg caaacgcaaa cctgcctgct cccttcttca cccttcccca gctgaaggat
      721 tccttcagga atgtcggcct gaaccgaagt agtgatttgg tggctctgtc cggaggccac
      781 acattcggca agagtcagtg caggttcatc atggacagac tgtataactt ttccaacacc
      841 ggcttgcctg
//
