LOCUS       sequence-153640-         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A118 attB-attP. GFP will express if sites recombine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR239
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(266..352)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(455..475)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gccctaggtc tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtataat
       61 atattcaggg agaccacaac ggtttccctc tacaaataat tttgtttaac tttttaatta
      121 acggccgaac ttttcggatc aagctatgaa ggacgcaaag agggaactaa acacttgcat
      181 cgatcgtacg tccggcaatt aaaaaagcgg ctaaccacgc cgcttttttt acgtctgcag
      241 gcgccactcg attctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac
      301 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctct
      361 agcacgcgta ccatgggatc ccccgggctg caggaattcg atatcaagct tttatttgta
      421 gagatcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg
      481 gtctctcttt tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc
      541 tggtaaaagg acag
//
