LOCUS       sequence-153653-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible 
            expression of Gateway cloned elements (constitutive rtTA and 
            neomycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen
            K
  TITLE     Inducible Transgene Expression in Human iPS Cells Using Versatile 
            All-in-One piggyBac Transposons.
  JOURNAL   Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
  PUBMED    26025620
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1357:111-31. doi"; pages: " 
            10.1007/7651_2015_251"
COMMENT     Sequence Label: PB-TA-ERN
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(87..93,94..111,112..117))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(142..266)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(345..369)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    391..409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    428..446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    465..483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    502..520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    539..557
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    576..594
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    613..631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    650..668
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
       61 cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc
      121 tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta
      181 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
      241 cgttcagctt ttttgtacaa acttgtgatg ccgccaccgc ggaggctgga tcggtcccgg
      301 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
      361 aaacgagctc tgcttatata gggggtacct tctctatcac tgatagggag ctcgagacgt
      421 gtcgacttct ctatcactga tagggagacg ttctagacgt accttctcta tcactgatag
      481 ggagctcgag acgtgtcgac ttctctatca ctgataggga gacgttctag acgtaccttc
      541 tctatcactg atagggagct cgagacgtgt cgacttctct atcactgata gggagacgtt
      601 ctagacgtac cttctctatc actgataggg agctcgagac gtgtcgactt ctctatcact
      661 gatagggaga cgtgcggcca gcttggatcc ctcgagttaa ttaacgagag cataatattg
      721 atatgtgcca aagttgtttc tgactgacta ataagtataa tttgtttcta ttatgtatag
      781 gttaagctaa ttacttattt tataatacaa catgactgtt tttaaagtac aaaataagtt
      841 tattt
//
