LOCUS       sequence-153657-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible 
            expression of Gateway cloned elements (inducible mCherry and 
            constitutive rtTA and neomycin resistance) aka. KW111.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen
            K
  TITLE     Inducible Transgene Expression in Human iPS Cells Using Versatile 
            All-in-One piggyBac Transposons.
  JOURNAL   Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
  PUBMED    26025620
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1357:111-31. doi"; pages: " 
            10.1007/7651_2015_251"
COMMENT     Sequence Label: PB-TAC-ERN
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    54..606
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    56..623
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(216..235)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     445..462
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cttgatgatg gccatgttat cctcctcgcc cttgctcacc atggttgtgg ccatattatc
       61 atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta
      121 acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat
      181 ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga
      241 ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg
      301 tatcttatac acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct
      361 gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt
      421 ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa
      481 tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata
      541 tggtggaaaa taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag
      601 taacgttagg ggggggggag ggaga
//
