LOCUS       sequence-153659-         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Joesch M, Mankus D, Yamagata M, Shahbazi A, Shalek R, Suissa-Peleg 
            A, Meister M, Lichtman JW, Scheirer WJ, Sanes JR
  TITLE     Reconstruction of genetically identified neurons imaged by 
            serial-section electron microscopy.
  JOURNAL   Elife. 2016 Jul 7;5. pii: e15015. doi: 10.7554/eLife.15015.
  PUBMED    27383271
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-07-7"; volume: "5. pii: e15015. doi"; pages: " 
            10.7554/eLife.15015"
COMMENT     Sequence Label: AAV-CAG-DIO-APEX2NES
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(20..53)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(104..137)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(152..178)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 ctctagagtc gactccggaa taacttcgta taggatactt tatacgaagt tatgcagaat
       61 ggtagctgga ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat
      121 acattatacg aagttatggc gcgccctagt ccagggtcag gcgctccagg ggtggcagct
      181 gcagggcgtc ggcaaatccc agttctgaca gcttctgatg tgcttctgcg taatctgcga
      241 aaaaagcatc ctcgtcggct gcatacttat cgaccagagg tctgaacacg ggatcggaca
      301 gcagggcttt gtcgctgggc agctgcagca gcccttcctt ctctccactc agcagttcag
      361 taaagtaaga attgtcgaaa atcagggggt tgcttgtcca gggtccctcg aagcctgacc
      421 tttccttatg agcggcgcca atggtgtgcc ctccagacag tgcgacgata tcctggtcag
      481 tcaggcccat agctttccca aacacatctc gcagatggtc ggagcccttt gtcggatctg
      541 gcaggcgccc ctctggaggt ggttctggtt tgtcctcccg tcctgggtgg aaaggcacct
      601 tggggccccc agtgacctcc acagcgacca ctcctgccag ctgataaaaa tcggcgta
//
