LOCUS       sequence-153661-         343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            T7 (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB425
FEATURES             Location/Qualifiers
     source          1..343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(24..30,31..48,49..54))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(91..215)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(241..258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(241..257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(250..272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
       61 ggtgcctaat gcggcccctc gaggggccgc catagtgact ggatatgttg tgttttacag
      121 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
      181 tacgtttctc gttcagcttt tttgtacaaa cttgttgata actctagacc caagcttggc
      241 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      301 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaa
//
