LOCUS       sequence-153662-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible 
            expression of Gateway cloned elements (inducible GFP and 
            constitutive rtTA and neomycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen
            K
  TITLE     Inducible Transgene Expression in Human iPS Cells Using Versatile 
            All-in-One piggyBac Transposons.
  JOURNAL   Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
  PUBMED    26025620
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1357:111-31. doi"; pages: " 
            10.1007/7651_2015_251"
COMMENT     Sequence Label: PB-TAG-ERN
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(22..30,31..33,34..608)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(195..214)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     424..441
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ctcctcgccc ttgctcacca tggttgtggc catattatca tcgtgttttt caaaggaaaa
       61 ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa
      121 agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct
      181 tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac
      241 tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt
      301 ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag
      361 acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac
      421 cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc
      481 caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga
      541 caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg gggggggagg
      601 gagagggggg gg
//
