LOCUS       sequence-153663-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible 
            expression of Gateway cloned elements (inducible GFP and 
            constitutive rtTA and neomycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen
            K
  TITLE     Inducible Transgene Expression in Human iPS Cells Using Versatile 
            All-in-One piggyBac Transposons.
  JOURNAL   Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
  PUBMED    26025620
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1357:111-31. doi"; pages: " 
            10.1007/7651_2015_251"
COMMENT     Sequence Label: PB-TAG-ERN
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(88..94,95..112,113..118))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(143..267)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(347..371)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    393..411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    430..448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    467..485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    504..522
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    541..559
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    578..596
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    615..633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    652..670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
       61 tcgacggatc ctaactcaaa atccacacat tatacgagcc ggaagcataa agtgtaaagc
      121 ctggggtgcc taatgcggcc gccatagtga ctggatatgt tgtgttttac agtattatgt
      181 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      241 tcgttcagct tttttgtaca aacttgtgat ggccgccacc gcggaggctg gatcggtccc
      301 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
      361 ctaaacgagc tctgcttata tagggggtac cttctctatc actgataggg agctcgagac
      421 gtgtcgactt ctctatcact gatagggaga cgttctagac gtaccttctc tatcactgat
      481 agggagctcg agacgtgtcg acttctctat cactgatagg gagacgttct agacgtacct
      541 tctctatcac tgatagggag ctcgagacgt gtcgacttct ctatcactga tagggagacg
      601 ttctagacgt accttctcta tcactgatag ggagctcgag acgtgtcgac ttctctatca
      661 ctgataggga gacgtgcggc cagcttggat ccctcgagtt aattaacgag agcataatat
      721 tgatatgtgc caaagttgtt tctgactgac taataagtat aatttgtttc tattatgtat
      781 aggttaagct aattacttat t
//
