LOCUS       sequence-153669-         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible 
            expression of Gateway cloned elements (inducible mCherry and 
            constitutive rtTA and puromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen
            K
  TITLE     Inducible Transgene Expression in Human iPS Cells Using Versatile 
            All-in-One piggyBac Transposons.
  JOURNAL   Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
  PUBMED    26025620
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1357:111-31. doi"; pages: " 
            10.1007/7651_2015_251"
COMMENT     Sequence Label: PB-TAC-ERP2
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(47..55,56..58,59..633)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(220..239)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     449..466
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtt gtggccatat
       61 tatcatcgtg tttttcaaag gaaaaccacg tccccgtggt tcggggggcc tagacgtttt
      121 tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc agatcccata
      181 caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga ggagagccat
      241 ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc cgcctttgca
      301 ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg tggggggttc
      361 cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc cagaggaact
      421 gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga aagaccccta
      481 ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc aaaagacggc
      541 aatatggtgg aaaataacat atagacaaac gcacaccggc cttattccaa gcggcttcgg
      601 ccagtaacgt tagggggggg ggagggagag ggggg
//
