LOCUS       sequence-153678-         268 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            4xMyc (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB516
FEATURES             Location/Qualifiers
     source          1..268
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(29..35,36..53,54..59))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(96..220)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(246..263)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(246..262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
       61 cctggggtgc ctaatgcggc ccctcgaggg gccgccatag tgactggata tgttgtgttt
      121 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      181 cattttacgt ttctcgttca gcttttttgt acaaacttgt tgataactct agacccaagc
      241 ttggcactgg ccgtcgtttt acaacgtc
//
