LOCUS       sequence-153679-         293 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for expression of gene by 
            CaMV35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB402
FEATURES             Location/Qualifiers
     source          1..293
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(32..38,39..56,57..62))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(99..223)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(247..293)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
       61 aagcctgggg tgcctaatgc ggcccctcga ggggccgcca tagtgactgg atatgttgtg
      121 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      181 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgttgataac tctagagtcc
      241 cccgtgttct ctccaaatga aatgaacttc cttatataga ggaagggtct tgc
//
