LOCUS       sequence-153691-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: ppTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP273
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(588..834)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(876..902)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctcaccatg aattcttttg ttgttgagtg aagcgagtga cggaacggta aaatgtaagt
       61 aacaaaagaa aaagagaacc agggggggga ggagagtatg tatttatacc gtacggcacc
      121 aggcgaaaag ctataaacaa acctttttcg cggtatattt gtttatattt cctattttaa
      181 actcaaaatc tgccctaggc gcgcccatag ggtatcctca ctccgattga tgaagagacc
      241 cttctggttc caatcctcac tccgattgat gtgacgtcaa tgggtggagt atatcctcac
      301 tccgattgat gagtgctctc gatgacttcg atatcctcac tccgattgat gaactccgcc
      361 ccattgacgc aaatcctcac tccgattgat ggtggaacag cccaccctgt acatcctcac
      421 tccgattgat gtgcggcctc gagaagcagc tgaatcctca ctccgattga tgactggacc
      481 gtcaccaatt ggagatcctc actccgattg atggtggtaa actcgcaata tcctcactcc
      541 gattgatgaa ttgatctgcc gcgatgactt ctatatgata tgctagctct cacttaatct
      601 tctgtactct gaagaggagt gggaaatacc aagaaaaaca tcaaactcga atgattttcc
      661 caaaccccta ccacaagata ttcatcagct gcgagatagg ctgatcagga gcaagctcgt
      721 acgagaagaa acaaaatgac aaaaaaaatc ctatactata taggttacaa ataaaaaagt
      781 atcaaaaatg aagcctgcat ctctcaggca aatggcattc tgacatcctc ttgattagaa
      841 tctagcaaga ccggtctacc gatggtacct caagaagcgt agtccggaac gtcgtacggg
      901 tagttaatta acatatcgag atcgaa
//
