LOCUS       sequence-153692-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: scTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP263
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..83
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    86..104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(616..862)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(904..930)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgctcaccat gaattctgtg ttttgatagt tgttcaattg attgaaatag ggacaaataa
       61 attatctcta tcactgatag ggagatctct atcactgata gggaaattta aagtctttgg
      121 gtcaggagaa accaaaattg ggaaaggtgt tcgcctttta tattcgattc tggtggtttc
      181 caataatctc atgacatgcg tccgcccgct attattgcca gcgacggcgc gcccataggg
      241 tatcctcact ccgattgatg aagagaccct tctggttcca atcctcactc cgattgatgt
      301 gacgtcaatg ggtggagtat atcctcactc cgattgatga gtgctctcga tgacttcgat
      361 atcctcactc cgattgatga actccgcccc attgacgcaa atcctcactc cgattgatgg
      421 tggaacagcc caccctgtac atcctcactc cgattgatgt gcggcctcga gaagcagctg
      481 aatcctcact ccgattgatg actggaccgt caccaattgg agatcctcac tccgattgat
      541 ggtggtaaac tcgcaatatc ctcactccga ttgatgaatt gatctgccgc gatgacttct
      601 atatgatatg ctagctctca cttaatcttc tgtactctga agaggagtgg gaaataccaa
      661 gaaaaacatc aaactcgaat gattttccca aacccctacc acaagatatt catcagctgc
      721 gagataggct gatcaggagc aagctcgtac gagaagaaac aaaatgacaa aaaaaatcct
      781 atactatata ggttacaaat aaaaaagtat caaaaatgaa gcctgcatct ctcaggcaaa
      841 tggcattctg acatcctctt gattagaatc tagcaagacc ggtctaccga tggtacctca
      901 agaagcgtag tccggaacgt cgtacgggta gttaattaac atatcgagat cga
//
