LOCUS       sequence-153694-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (puromycin selection) with multiple
            cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-MCS
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    110..639
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ctgtgggaag gggcaggaga gccaggggca tgagatggtg gacgaggaag ggggacaggg
       61 aagcctgagc gcctctcctg ggcttgccaa ggactcaaac ccagaagccc agagcagggc
      121 cttagggaag cgggaccctg ctctgggcgg aggaatatgt cccagatagc actggggact
      181 ctttaaggaa agaaggatgg agaaagagaa agggagtaga ggcggccacg acctggtgaa
      241 cacctaggac gcaccattct cacaaaggga gttttccaca cggacacccc cctcctcacc
      301 acagccctgc caggacgggg ctggctactg gccttatctc acaggtaaaa ctgacgcacg
      361 gaggaacaat ataaattggg gactagaaag gtgaagagcc aaagttagaa ctcaggacca
      421 acttattctg attttgtttt tccaaactgc ttctcctctt gggaagtgta aggaagctgc
      481 agcaccagga tcagtgaaac gcaccagaca gccgcgtcag agcagctcag gttctgggag
      541 agggtagcgc agggtggcca ctgagaaccg ggcaggtcac gcatcccccc cttccctccc
      601 accccctgcc aagctctccc tcccaggatc ctctctggct ccatcgtaag caaaccttag
      661 aggttctggc aaggagagag atggctccag gaaatggggg tgtgtcacca gataaggaat
      721 ctgcctaaca ggaggtgggg gttagaccca atatcaggag actaggaagg aggaggccta
      781 aggatggggc ttttctgtca aagcttagta ctgtcgactt ctagatttaa t
//
