LOCUS       sequence-153695-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: GAP6, minimal Promoter: GAP6. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP262
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..77
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(610..856)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(898..924)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatgaattc tgtgttttga tagttgttca attgattgaa atagggacaa ataaattatc
       61 tctatcactg atagggagat ctctatcact gatagggaaa tttaaagtct ttgggtcagg
      121 agaaaccaaa attgggaaag gtgttcgcct tttatattcg attctggtgg tttccaataa
      181 tctcatgaca tgcgtccgcc cgctattatt gccagcgacg gcgcgcccat agggtatcct
      241 cactccgatt gatgaagaga cccttctggt tccaatcctc actccgattg atgtgacgtc
      301 aatgggtgga gtatatcctc actccgattg atgagtgctc tcgatgactt cgatatcctc
      361 actccgattg atgaactccg ccccattgac gcaaatcctc actccgattg atggtggaac
      421 agcccaccct gtacatcctc actccgattg atgtgcggcc tcgagaagca gctgaatcct
      481 cactccgatt gatgactgga ccgtcaccaa ttggagatcc tcactccgat tgatggtggt
      541 aaactcgcaa tatcctcact ccgattgatg aattgatctg ccgcgatgac ttctatatga
      601 tatgctagct ctcacttaat cttctgtact ctgaagagga gtgggaaata ccaagaaaaa
      661 catcaaactc gaatgatttt cccaaacccc taccacaaga tattcatcag ctgcgagata
      721 ggctgatcag gagcaagctc gtacgagaag aaacaaaatg acaaaaaaaa tcctatacta
      781 tataggttac aaataaaaaa gtatcaaaaa tgaagcctgc atctctcagg caaatggcat
      841 tctgacatcc tcttgattag aatctagcaa gaccggtcta ccgatggtac ctcaagaagc
      901 gtagtccgga acgtcgtacg ggtagttaat taacatatcg agatcga
//
