LOCUS       sequence-153699-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Joesch M, Mankus D, Yamagata M, Shahbazi A, Shalek R, Suissa-Peleg 
            A, Meister M, Lichtman JW, Scheirer WJ, Sanes JR
  TITLE     Reconstruction of genetically identified neurons imaged by 
            serial-section electron microscopy.
  JOURNAL   Elife. 2016 Jul 7;5. pii: e15015. doi: 10.7554/eLife.15015.
  PUBMED    27383271
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-07-7"; volume: "5. pii: e15015. doi"; pages: " 
            10.7554/eLife.15015"
COMMENT     Sequence Label: AAV-CAG-DIO-APEX2NES
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(131..164)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             181..222
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag
       61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg
      121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc cgccaccatg
      181 ggcaagccca tccccaaccc cctgctgggc ctggacagca ccaccggtgg gaaatcatac
      241 ccaacagtgt ccgcagacta ccaggatgcc gtggagaaag ccaagaagaa gctgagaggg
      301 tttatcgcag aaaagaggtg cgcacctctg atgctgagac tggctttcca cagcgcaggc
      361 acctttgaca agggaaccaa aacaggcgga ccctttggaa caatcaagca ccctgctgaa
      421 ctggcacata gtgccaacaa tgggctggac atcgccgtgc ggctgctgga acctctgaaa
      481 gcagagttcc caattctgtc ctacgccgat ttttatcagc tggcaggagt ggtcgctgtg
      541 gaggtcactg ggggccccaa ggtgcctttc cacccaggac gggaggacaa accagaacca
      601 cctccagagg ggcgcctgcc agatccgaca aagggctccg accatctgcg agatgtgttt
      661 gggaaagcta tgggcctgac tgaccaggat atcgtcgcac tgtctggagg gcacaccatt
      721 ggcgccgctc ataaggaaag gtcaggcttc gagggaccct ggacaagcaa ccccctgatt
      781 ttcgacaatt cttactttac tgaactgctg agtggagaga aggaagggct gctgcagctg
      841 cccagcgaca aagccctgct gtccgatccc gtgttcagac ctctggtcga taagtatgca
      901 gccgacgagg atgctttttt cgcagattac gcagaagcac atcag
//
