LOCUS       sequence-153700-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: ppTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP229
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..81
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    84..102
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(614..860)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 ctcaccatga attctgtgtt ttgatagttg ttcaattgat tgaaataggg acaaataaat
       61 tatctctatc actgataggg agatctctat cactgatagg gaaatttaaa gtctttgggt
      121 caggagaaac caaaattggg aaaggtgttc gccttttata ttcgattctg gtggtttcca
      181 ataatctcat gacatgcgtc cgcccgctat tattgccagc gacggcgcgc ccatagggta
      241 tcctcactcc gattgatgaa gagacccttc tggttccaat cctcactccg attgatgtga
      301 cgtcaatggg tggagtatat cctcactccg attgatgagt gctctcgatg acttcgatat
      361 cctcactccg attgatgaac tccgccccat tgacgcaaat cctcactccg attgatggtg
      421 gaacagccca ccctgtacat cctcactccg attgatgtgc ggcctcgaga agcagctgaa
      481 tcctcactcc gattgatgac tggaccgtca ccaattggag atcctcactc cgattgatgg
      541 tggtaaactc gcaatatcct cactccgatt gatgaattga tctgccgcga tgacttctat
      601 atgatatgct agctctcact taatcttctg tactctgaag aggagtggga aataccaaga
      661 aaaacatcaa actcgaatga ttttcccaaa cccctaccac aagatattca tcagctgcga
      721 gataggctga tcaggagcaa gctcgtacga gaagaaacaa aatgacaaaa aaaatcctat
      781 actatatagg ttacaaataa aaaagtatca aaaatgaagc ctgcatctct caggcaaatg
      841 gcattctgac atcctcttga ttagaatcta gcaagaccgg tctac
//
