LOCUS       sequence-153707-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (puromycin selection) and 
            constitutive GFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-CAG-GFP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    121..650
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ccaggaaccc ctgtagggaa ggggcaggag agccaggggc atgagatggt ggacgaggaa
       61 gggggacagg gaagcctgag cgcctctcct gggcttgcca aggactcaaa cccagaagcc
      121 cagagcaggg ccttagggaa gcgggaccct gctctgggcg gaggaatatg tcccagatag
      181 cactggggac tctttaagga aagaaggatg gagaaagaga aagggagtag aggcggccac
      241 gacctggtga acacctagga cgcaccattc tcacaaaggg agttttccac acggacaccc
      301 ccctcctcac cacagccctg ccaggacggg gctggctact ggccttatct cacaggtaaa
      361 actgacgcac ggaggaacaa tataaattgg ggactagaaa ggtgaagagc caaagttaga
      421 actcaggacc aacttattct gattttgttt ttccaaactg cttctcctct tgggaagtgt
      481 aaggaagctg cagcaccagg atcagtgaaa cgcaccagac agccgcgtca gagcagctca
      541 ggttctggga gagggtagcg cagggtggcc actgagaacc gggcaggtca cgcatccccc
      601 ccttccctcc caccccctgc caagctctcc ctcccaggat cctctctggc tccatcgtaa
      661 gcaaacctta gaggttctgg caaggagaga gatggctcca ggaaatgggg gtgtgtcacc
      721 agataaggaa tctgcctaac aggaggtggg ggttagaccc aatatcagga gactaggaag
      781 gaggaggcct aaggatgggg cttttctgtc accaatcctg
//
