LOCUS       sequence-153708-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (puromycin selection) and 
            constitutive GFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-CAG-GFP
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    187..688
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 cctttgcttt ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc
       61 ttgtggcctg ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt
      121 ccgtcttcct ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc
      181 tttccggagc acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc
      241 cgtgtctggg tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact
      301 cgctgggttc ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg
      361 gccttctccg acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat
      421 caccgttttt ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat
      481 cctcttgctt tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc
      541 ctttcatttg ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag
      601 ccccctgtca tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc
      661 gggcccctat gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg
      721 agctgggacc accttatatt ccca
//
