LOCUS       sequence-153712-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (puromycin selection) and 
            constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-CAG-mCh
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    158..659
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc tacggctggc
       61 ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct cttccccttg
      121 ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc tcggcgctgc
      181 accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg ggcatctctc
      241 ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct tctccttctg
      301 gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc tcccttgcgt
      361 cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac cccaaagtac
      421 cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct ggacaccccg
      481 ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc cctacccccc
      541 ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt ccaggggtcc
      601 gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc aggacagcat
      661 gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat tcccagggcc
      721 ggttaatgtg gctctggttc tgggtacttt tatctgtccc
//
