LOCUS       sequence-153713-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (puromycin selection) and 
            constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-CAG-mCh
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    106..635
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 gggaaggggc aggagagcca ggggcatgag atggtggacg aggaaggggg acagggaagc
       61 ctgagcgcct ctcctgggct tgccaaggac tcaaacccag aagcccagag cagggcctta
      121 gggaagcggg accctgctct gggcggagga atatgtccca gatagcactg gggactcttt
      181 aaggaaagaa ggatggagaa agagaaaggg agtagaggcg gccacgacct ggtgaacacc
      241 taggacgcac cattctcaca aagggagttt tccacacgga cacccccctc ctcaccacag
      301 ccctgccagg acggggctgg ctactggcct tatctcacag gtaaaactga cgcacggagg
      361 aacaatataa attggggact agaaaggtga agagccaaag ttagaactca ggaccaactt
      421 attctgattt tgtttttcca aactgcttct cctcttggga agtgtaagga agctgcagca
      481 ccaggatcag tgaaacgcac cagacagccg cgtcagagca gctcaggttc tgggagaggg
      541 tagcgcaggg tggccactga gaaccgggca ggtcacgcat cccccccttc cctcccaccc
      601 cctgccaagc tctccctccc aggatcctct ctggctccat cgtaagcaaa ccttagaggt
      661 tctggcaagg agagagatgg ctccaggaaa tgggggtgtg tcaccagata aggaatctgc
      721 ctaacaggag gtgggggtta gacccaatat caggagacta ggaaggagga ggcctaagga
      781 tggggctttt ctgtcaaagc ttagtactgt cgacttctag atttaatta
//
