LOCUS       sequence-153715-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (puromycin selection) and 
            constitutive GFP-luciferase fusion protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-CAG-GFPluc2
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg ggtcacctct acggctggcc
       61 cagatccttc cctgccgcct ccttcaggtt ccgtcttcct ccactccctc ttccccttgc
      121 tctctgctgt gttgctgccc aaggatgctc tttccggagc acttccttct cggcgctgca
      181 ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg tcctctccgg gcatctctcc
      241 tccctcaccc aaccccatgc cgtcttcact cgctgggttc ccttttcctt ctccttctgg
      301 ggcctgtgcc atctctcgtt tcttaggatg gccttctccg acggatgtct cccttgcgtc
      361 ccgcctcccc ttcttgtagg cctgcatcat caccgttttt ctggacaacc ccaaagtacc
      421 ccgtctccct ggctttagcc acctctccat cctcttgctt tctttgcctg gacaccccgt
      481 tctcctgtgg attcgggtca cctctcactc ctttcatttg ggcagctccc ctacccccct
      541 tacctctcta gtctgtgcta gctcttccag ccc
//
