LOCUS       sequence-153717-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (puromycin selection) and 
            constitutive GFP-luciferase fusion protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-CAG-GFPluc2
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    111..640
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 cctgtgggaa ggggcaggag agccaggggc atgagatggt ggacgaggaa gggggacagg
       61 gaagcctgag cgcctctcct gggcttgcca aggactcaaa cccagaagcc cagagcaggg
      121 ccttagggaa gcgggaccct gctctgggcg gaggaatatg tcccagatag cactggggac
      181 tctttaagga aagaaggatg gagaaagaga aagggagtag aggcggccac gacctggtga
      241 acacctagga cgcaccattc tcacaaaggg agttttccac acggacaccc ccctcctcac
      301 cacagccctg ccaggacggg gctggctact ggccttatct cacaggtaaa actgacgcac
      361 ggaggaacaa tataaattgg ggactagaaa ggtgaagagc caaagttaga actcaggacc
      421 aacttattct gattttgttt ttccaaactg cttctcctct tgggaagtgt aaggaagctg
      481 cagcaccagg atcagtgaaa cgcaccagac agccgcgtca gagcagctca ggttctggga
      541 gagggtagcg cagggtggcc actgagaacc gggcaggtca cgcatccccc ccttccctcc
      601 caccccctgc caagctctcc ctcccaggat cctctctggc tccatcgtaa gcaaacctta
      661 gaggttctgg caaggagaga gatggctcca ggaaatgggg gtgtgtcacc agataaggaa
      721 tctgcctaac aggaggtggg ggttagaccc aatatcagga gactaggaag gaggaggcct
      781 aaggatgggg cttttctgtc aaagcttagt actgtcgact
//
