LOCUS       sequence-153727-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A bicistronic lentiviral vector used to stably express cDNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C, 
            Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J
  TITLE     Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins 
            engineered from human components.
  JOURNAL   J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub
            2009 Sep 8.
  PUBMED    19741300
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI39354"; 
            journalName: "J Clin Invest"; date: "2009-10"; volume: "119"; issue:
            "10"; pages: "3035-47"
COMMENT     Sequence Label: pFUPI
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             165..764
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(165..184)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     661..681
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(940..957)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
       61 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
      121 ttagacgaag cttgggctgc aggtcgactc tagctagagc caccatgacc gagtacaagc
      181 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
      241 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
      301 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
      361 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
      421 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
      481 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
      541 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
      601 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
      661 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
      721 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagaattc cgcccctctc
      781 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
      841 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
      901 gtcctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
      961 gtctgttgaa tgtcgtgaag gaagcagttc ctctgga
//
