LOCUS       sequence-153728-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A bicistronic lentiviral vector used to stably express cDNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C, 
            Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J
  TITLE     Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins 
            engineered from human components.
  JOURNAL   J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub
            2009 Sep 8.
  PUBMED    19741300
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI39354"; 
            journalName: "J Clin Invest"; date: "2009-10"; volume: "119"; issue:
            "10"; pages: "3035-47"
COMMENT     Sequence Label: pFUPI
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    199..787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(252..272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(670..681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(790..806)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(791..807)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
       61 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      121 ctttgaaaaa cacgatgata accggtggtc tagaggatcc gttaacggcg cgccgaattc
      181 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
      241 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
      301 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
      361 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
      421 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
      481 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
      541 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
      601 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
      661 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
      721 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
      781 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
      841 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
      901 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
      961 cttagccact ttt
//
