LOCUS       sequence-153737-       10958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding full length UBR5 W1235L (∆UBR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, 
            Stokke T, Patzke S, Saunders DN
  TITLE     The E3 ubiquitin ligase UBR5 regulates centriolar satellite 
            stability and primary cilia.
  JOURNAL   Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 
            10.1091/mbc.E17-04-0248.
  PUBMED    29742019
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248."
COMMENT     Sequence Label: pENTR221 UBR5 ∆UBR
FEATURES             Location/Qualifiers
     source          1..10958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(9083..9182)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9199..9218)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9200..9218)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9223..9239)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9223..9239)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9341..9360)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9352..10161
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9419..9438)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10308..10896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10797..10816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc cggaccatga cgtccatcca tttcgtggtt cacccgctgc cgggcaccga
      721 ggaccagctc aatgacaggt tacgagaagt ttctgagaag ctgaacaaat ataatttaaa
      781 cagccacccc cctttgaatg tattggaaca ggctactatt aaacagtgtg tggtgggacc
      841 aaatcatgct gcctttcttc ttgaggatgg tagagtttgc aggattggtt tttcagtaca
      901 gccagacaga ttggaattgg gtaaacctga taataatgat gggtcaaagt tgaacagcaa
      961 ctcgggggca gggaggacgt caaggcctgg taggacaagc gactctccat ggtttctctc
     1021 aggttctgag actctaggca ggctggcagg caacacctta ggaagccgct ggagttctgg
     1081 agtgggtgga agtggtggag gatcctctgg taggtcatca gctggagctc gagattcccg
     1141 ccggcagact cgagttattc ggacaggacg ggatcgaggg tctgggcttt tgggcagtca
     1201 gccccagcca gttattccag catctgtcat tccagaggag ctgatttcac aggcccaagt
     1261 tgttttacaa ggcaaatcca gaagtgtcat tattcgagaa cttcagagaa caaatcttga
     1321 tgtgaacctt gctgtaaata atttacttag ccgggatgat gaagatggag atgatgggga
     1381 tgatacagcc agcgaatctt atttgcctgg agaggatctt atgtctctcc ttgatgccga
     1441 cattcattct gcccacccaa gtgtcattat tgatgcagat gccatgtttt ctgaagacat
     1501 tagctatttt ggttaccctt cttttcgtcg ttcatcactt tccaggctag gctcatctcg
     1561 agttctcctt cttcccttag agagagactc tgagctgttg cgtgaacgtg aatccgtttt
     1621 acgtttacgt gaacgaaggt ggcttgatgg agcctcattt gataatgaaa ggggttctac
     1681 cagcaaggaa ggagagccaa acttggataa gaagaataca cctgttcaaa gtccagtatc
     1741 tctaggagaa gatttgcagt ggtggcctga taaggatgga acaaaattca tctgtattgg
     1801 ggctctgtat tctgaacttc tggctgtcag cagtaaagga gaactttatc agtggaaatg
     1861 gagtgaatct gagccttaca gaaatgccca gaatccttca ttacatcatc cacgagcaac
     1921 atttttgggg ttaaccaatg aaaagatagt cctcctgtct gcaaatagca taagagcaac
     1981 tgtagctaca gaaaataaca aggttgctac atgggtggat gaaactttaa gttctgtggc
     2041 ttctaaatta gagcacactg ctcagactta ctctgaactt caaggagagc ggatagtttc
     2101 tttacattgc tgtgcccttt acacctgcgc tcagctggaa aacagtttat attggtgggg
     2161 tgtagttcct tttagtcaaa ggaagaaaat gttagagaaa gctagagcaa aaaataaaaa
     2221 gcctaaatcc agtgctggta tttcttcaat gccgaacatc actgttggta cccaggtatg
     2281 cttgagaaat aatcctcttt atcatgctgg agcagttgca ttttcaatta gtgctgggat
     2341 tcctaaagtt ggtgtcttaa tggagtcagt ttggaatatg aatgacagct gtagatttca
     2401 acttagatct cctgaaagct tgaaaaacat ggaaaaagct agcaaaacta ctgaagctaa
     2461 gcctgaaagt aagcaggagc cagtgaaaac agaaatgggt cctccaccat ctccagcatc
     2521 cacgtgtagt gatgcatcct caattgccag cagtgcatca atgccataca aacgacgacg
     2581 gtcaacccct gcaccaaaag aagaggaaaa ggtgaatgaa gagcagtggt ctcttcggga
     2641 agtggttttt gtggaagatg tcaagaatgt tcctgttggc aaggtgctaa aagtagatgg
     2701 tgcctatgtt gctgtaaaat ttccaggaac ctccagtaat actaactgtc agaacagctc
     2761 tggtccagat gctgaccctt cttctctcct gcaggattgt aggttactta gaattgatga
     2821 attgcaggtt gtcaaaactg gtggaacacc gaaggttccc gactgtttcc aaaggactcc
     2881 taaaaagctt tgtatacctg aaaaaacaga aatattagca gtgaatgtag attccaaagg
     2941 tgttcatgct gttctgaaga ctggaaattg ggtgcgatac tgtatctttg atcttgctac
     3001 aggaaaagca gaacaggaaa ataattttcc tacaagcagc attgctttcc ttggtcagaa
     3061 tgagaggaat gtagccattt tcactgctgg acaggaatct cccattattc ttcgagatgg
     3121 aaatggtacc atctacccaa tggccaaaga ttgcatggga ggaataaggg atcccgattg
     3181 gctggatctt ccacctatta gtagtcttgg aatgggtgtg cattctttaa taaatcttcc
     3241 tgccaattca acaatcaaaa agaaagctgc tgttatcatc atggctgtag agaaacaaac
     3301 cttaatgcaa cacattctgc gctgtgacta tgaggcctgt cgacaatatc taatgaatct
     3361 tgagcaagcg gttgttttag agcagaatct acagatgctg cagacattca tcagccacag
     3421 atgtgatgga aatcgaaata ttttgcatgc ttgtgtatca gtttgctttc caaccagcaa
     3481 taaagaaact aaagaagaag aggaagcgga gcgttctgaa agaaatacat ttgcagaaag
     3541 gctttctgct gttgaggcca ttgcaaatgc aatatcagtt gtttcaagta atggcccagg
     3601 taatcgggct ggatcatcaa gtagccgaag tttgagatta cgggaaatga tgagacgttc
     3661 gttgagagca gctggtttgg gtagacatga agctggagct tcatccagtg accaccagga
     3721 tccagtttca ccccccatag ctccccctag ttgggttcct gaccctcctg cgatggatcc
     3781 tgatggtgac attgatttta tcctggcccc cgctgtggga tctcttacca cagcagcaac
     3841 cggtactggt caaggaccaa gcacctccac tattccaggt ccttccacag agccatctgt
     3901 agtagaatcc aaggatcgaa aggcgaatgc tcattttata ttgaaattgt tatgtgacag
     3961 tgtggttctc cagccctatc tacgagaact tctttctgcc aaggatgcaa gagggatgac
     4021 cccatttatg tcagctgtaa gtggccgagc ttatcctgct gcaattacca tcttagaaac
     4081 tgctcagaaa attgcaaaag ctgaaatatc ctcaagtgaa aaagaggaag atgtattcat
     4141 gggaatggtt tgcccatcag gtaccaaccc tgatgactct cctttatatg ttttatgttg
     4201 taatgacact tgcagtttta catggactgg agcagagcac attaaccagg atatttttga
     4261 gtgtcgaact tgtggcttgc tggagtcact gtgttgttgt acggaatgtg caagggtttg
     4321 tcataaaggt catgattgca aactcaaacg gacatcacca acagcctact gtgattgttt
     4381 ggagaaatgt aaatgtaaaa ctcttattgc tggacagaaa tctgctcgtc ttgatctact
     4441 ttatcgcctg ctcactgcta ctaatctggt tactctgcca aacagcaggg gagagcacct
     4501 cttactattc ttagtacaga cagtcgcaag gcagacggtg gagcattgtc aatacaggcc
     4561 acctcgaatc agggaagatc gtaaccgaaa aacagccagt cctgaagatt cagatatgcc
     4621 agatcatgat ttagagcctc caagatttgc ccagcttgca ttggagcgtg ttctacagga
     4681 ctggaatgcc ttgaaatcta tgattatgtt tgggtcgcag gagaataaag accctcttag
     4741 tgccagcagt agaataggcc atcttttgcc agaagagcaa gtatacctca atcagcaaag
     4801 tggcacaatt cggctggact gtttcactca ttgccttata gttaagtgta cagcagatat
     4861 tttgctttta gatactctac taggtacact agtgaaagaa ctccaaaaca aatatacacc
     4921 tggacgtaga gaagaagcta ttgctgtgac aatgaggttt ctacgttcag tggcaagagt
     4981 ttttgttatt ctgagtgtgg aaatggcttc atccaaaaag aaaaacaact ttattccaca
     5041 gccaattgga aaatgcaagc gtgtattcca agcattgcta ccttacgctg tggaagaatt
     5101 gtgcaacgta gcagagtcac tgattgttcc tgtcagaatg gggattgctc gtccaactgc
     5161 accatttacc ctggctagta ctagcataga tgccatgcag ggcagtgaag aattattttc
     5221 agtggaacca ctaccaccac gaccatcatc tgatcagtct agcagctcca gtcagtctca
     5281 gtcatcctac atcatcagga atccacagca gaggcgcatc agccagtcac agcccgttcg
     5341 gggcagagat gaagaacagg atgatattgt ttcagcagat gtggaagagg ttgaggtggt
     5401 ggagggtgtg gctggagaag aggatcatca tgatgaacag gaagaacacg gggaagaaaa
     5461 tgctgaggca gagggacaac atgatgagca tgatgaagac gggagtgata tggagctgga
     5521 cttgttagca gcagctgaaa cagaaagtga tagtgaaagt aaccacagca accaagataa
     5581 tgctagtggg cgcagaagcg ttgtcactgc agcaactgct ggttcagaag caggagcaag
     5641 cagtgttcct gccttctttt ctgaagatga ttctcaatcg aatgactcaa gtgattctga
     5701 tagcagtagt agtcagagtg acgacataga acaggagacc tttatgcttg atgagccatt
     5761 agaaagaacc acaaatagct cccatgccaa tggtgctgcc caagctcccc gttcaatgca
     5821 gtgggctgtc cgcaacaccc agcatcagcg agcagccagt acagcccctt ccagtacatc
     5881 tacaccagca gcaagttcag cgggtttgat ttatattgat ccttcaaact tacgccggag
     5941 tggtaccatc agtacaagtg ctgcagctgc agcagctgct ttggaagcta gcaacgccag
     6001 cagttaccta acatctgcaa gcagtttagc cagggcttac agcattgtca ttagacaaat
     6061 ctcggacttg atgggcctta ttcctaagta taatcaccta gtatactctc agattccagc
     6121 agctgtgaaa ttgacttacc aagatgcagt aaacttacag aactatgtag aagaaaagct
     6181 tattcccact tggaactgga tggtcagtat tatggattct actgaagctc aattacgtta
     6241 tggttctgca ttagcatctg ctggtgatcc tggacatcca aatcatcctc ttcacgcttc
     6301 tcagaattca gcgagaagag agaggatgac tgcgcgagaa gaagctagct tacgaacact
     6361 tgaaggcaga cgacgtgcca ccttgcttag cgcccgtcaa ggaatgatgt ctgcacgagg
     6421 agacttccta aattatgctc tgtctctaat gcggtctcat aatgatgagc attctgatgt
     6481 tcttccagtt ttggatgttt gctcattgaa gcatgtggca tatgtttttc aagcacttat
     6541 atactggatt aaggcaatga atcagcagac aacattggat acacctcaac tagaacgcaa
     6601 aaggacgcga gaactcttgg aactgggtat tgataatgaa gattcagaac atgaaaatga
     6661 tgatgacacc aatcaaagtg ctactttgaa tgataaggat gatgactctc ttcctgcaga
     6721 aactggccaa aaccatccat ttttccgacg ttcagactcc atgacattcc ttgggtgtat
     6781 acccccaaat ccatttgaag tgcctctggc tgaagccatc cccttggctg atcagccaca
     6841 tctgttgcag ccaaatgcta gaaaggagga tctttttggc cgtccaagtc agggtcttta
     6901 ttcttcatct gccagtagtg ggaaatgttt aatggaggtt acagtggata gaaactgcct
     6961 agaggttctt ccaacaaaaa tgtcttatgc tgccaatctg aaaaatgtaa tgaacatgca
     7021 aaaccggcaa aaaaaagaag gggaagaaca gcccgtgctg ccagaagaaa ctgagagttc
     7081 aaaaccaggg ccatctgctc atgatcttgc tgcacaatta aaaagtagct tactagcaga
     7141 aataggactt actgaaagtg aagggccacc tctcacatct ttcaggccac agtgtagctt
     7201 tatgggaatg gttatttccc atgatatgct gctaggacgt tggcgccttt ctttagaact
     7261 gttcggcagg gtattcatgg aagatgttgg agcagaacct ggatcaatcc taactgaatt
     7321 gggtggtttt gaggtaaaag aatcaaaatt ccgcagagaa atggaaaaac tgagaaacca
     7381 gcagtcaaga gatttgtcac tagaggttga tcgggatcga gatcttctca ttcagcagac
     7441 tatgaggcag cttaacaatc actttggtcg aagatgtgct actacaccaa tggctgtaca
     7501 cagagtaaaa gtcacattta aggatgagcc aggagagggc agtggtgtag cacgaagttt
     7561 ttatacagcc attgcacaag catttttatc aaatgaaaaa ttgccaaatc tagagtgtat
     7621 ccaaaatgcc aacaaaggca cccacacaag tttaatgcag agattaagga accgaggaga
     7681 gagagaccgg gaaagggaga gagaaaggga aatgaggagg agtagtggtt tgcgagcagg
     7741 ttctcggagg gaccgggata gagactttag aagacagctt tccatcgaca ctaggccctt
     7801 tagaccagcc tctgaaggga atcctagcga tgatcctgag cctttgccag cacatcggca
     7861 ggcacttgga gagaggcttt atcctcgtgt acaagcaatg caaccagcat ttgcaagtaa
     7921 aatcactggc atgttgttgg aattatcccc agctcagctg cttctccttc tagcaagtga
     7981 ggattctctg agagcaagag tggatgaggc catggaactc attattgcac atggacggga
     8041 aaatggagct gatagtatcc tggatcttgg attagtagac tcctcagaaa aggtacagca
     8101 ggaaaaccga aagcgccatg gctctagtcg aagtgtagta gatatggatt tagatgatac
     8161 agatgatggt gatgacaatg cccctttgtt ttaccaacct gggaaaagag gattttatac
     8221 tccaaggcct ggcaagaaca cagaagcaag gttgaattgt ttcagaaaca ttggcaggat
     8281 tcttggacta tgtctgttac agaatgaact ctgtcctatc acattgaata gacatgtaat
     8341 taaagtattg cttggtagaa aagtcaattg gcatgatttt gctttttttg atcctgtaat
     8401 gtatgagagt ttgcggcaac taatcctcgc gtctcagagt tcagatgctg atgctgtttt
     8461 ctcagcaatg gatttggcat ttgcaattga cctgtgtaaa gaagaaggtg gaggacaggt
     8521 tgaactcatt cctaatggtg taaatatacc agtcactcca cagaatgtat atgagtatgt
     8581 gcggaaatac gcagaacaca gaatgttggt agttgcagaa cagcccttac atgcaatgag
     8641 gaaaggtcta ctagatgtgc ttccaaaaaa ttcattagaa gatttaacgg cagaagattt
     8701 taggcttttg gtaaatggct gcggtgaagt caatgtgcaa atgctgatca gttttacctc
     8761 tttcaatgat gaatcaggag aaaatgctga gaagcttctg cagttcaagc gttggttctg
     8821 gtcaatagta gagaagatga gcatgacaga acgacaagat cttgtttact tttggacatc
     8881 aagcccatca ctgccagcca gtgaagaagg attccagcct atgccctcaa tcacaataag
     8941 accaccagat gaccaacatc ttcctactgc aaatacttgc atttctcgac tttacgtccc
     9001 actctattcc tctaaacaga ttctcaaaca gaaattgtta ctcgccatta agaccaagaa
     9061 ttttggtttt gtgtagccgc ggacccagct ttcttgtaca aagttggcat tataagaaag
     9121 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     9181 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     9241 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aataatatca
     9301 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     9361 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     9421 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     9481 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     9541 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     9601 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     9661 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     9721 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     9781 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     9841 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     9901 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     9961 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
    10021 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
    10081 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
    10141 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
    10201 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
    10261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10441 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10921 cggttcctgg ccttttgctg gccttttgct cacatgtt
//
