LOCUS       sequence-153743-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Flag-tagged mRAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Sfeir A, Kabir S, van Overbeek M, Celli GB, de Lange T
  TITLE     Loss of Rap1 induces telomere recombination in the absence of NHEJ 
            or a DNA damage signal.
  JOURNAL   Science. 2010 Mar 26;327(5973):1657-61. doi: 
            10.1126/science.1185100.
  PUBMED    20339076
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2010-03-26- 26"; volume: "327"; 
            issue: "5973"; pages: "1657-61"
COMMENT     Sequence Label: pLPC Flag-mRAP1
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcagccac catggactac aaagacgatg acgataaagg
      121 atccgcggag gcgatggatc tgggtaaaga ccccaatggg cccactcact cctccactct
      181 gttcgtgaga gaagacggca gcgccatgtc gttttacgtg cggcccagct cggccaagcg
      241 ccggctgtcg acgctcatcc tgcacggcgg cggcaccgtg tgtcgggtgc aggaacccgg
      301 agccgtgctt ctcgcccagc ccggggaggc gctggccgag gcttcgggcg acttcatctc
      361 cacgcagtac atcctagact gcgtggatcg caacgagaag ctggacctgg aggcctatcg
      421 gctgggcctg acggagcagg cgtccgatcc gaagcccggg gcttccaccg agggctccac
      481 ggaaccggag ccgcagcccc tgaccgggcg catcgcctac accgacgcgg aagatgtggc
      541 catcctgacc tacgtgaagg agaacgcccg ttcgcccagc tcggtcacag gcaatgcctt
      601 gtggaaagcg atggagaaga gctcgctcac gcagcactcc tggcagtcgc tcaaggaccg
      661 ctacctcaag cacctacggg gtcaggagca caagtacctg ctcgggaacg ccccggtcag
      721 cccgtcctcc cagaagctca aacggaaggc ggagcaggac cccgaggccg cggatagcgg
      781 agagccacag aacaagagag cgccagactt gcctgaggag gagtgtgtga aaggagagat
      841 caaggagaat ggagaggcag acaacaagct gtttgaggaa gccgctccgg agttcgga
//
