LOCUS       sequence-153748-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 4. Promoter scTEF1. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP87
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(11..415)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     protein_bind    535..584
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 ccatgaattc cttagattag attgctatgt ttctttctaa tgaacaagaa gtaaaaaaag
       61 ttgtaataga acaagaaaaa tgaaactgaa acttgagaaa ttgaagaccg tttattaact
      121 taaatatcaa tggaggtcac tgaaagagaa aaaaactaaa aaaaaaaatt tcaagaaaaa
      181 gaaacgtgat aaaaattttt attgcctttt tcgacgaaga aaaagaaacg aggcggtctc
      241 ttttttcttt tccaaacctt tagtacgggt aattaacgac accctagagg aagaaagagg
      301 gaaaatttag tatgctgtgc ttgggtgttt tgaagtggta cggcgatgcg cggagtccga
      361 gaaaatctgg aagagtaaaa aaggagtaga aacattttga agctatggtg tgtggctcga
      421 ggtgatggaa ccaatttagc tatatatagt taactaccgg ctcgatcatc tctgcctcca
      481 gcatagtcga agaagaattt tttttttctt gaggcttctg tcagcaagct tgtggcccgg
      541 atgatcctga cgacggagac cgccgtcgtc gacaagccgg ccgacgagga cgagcacggc
      601 cacggccaaa cgagctcaga tctgatctca tgaccaaaat cccttaacgt gagttttcgt
      661 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      721 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      781 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      841 caaatactgt
//
