LOCUS       sequence-153749-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 4. Promoter GAP2. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP160
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    280..298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcaccatgaa ttctgtgttt tgatagttgt tcaattgatt gaaataggga caaataaatt
       61 aaatttaaag tctttgggtc aggagaaacc aaaattggga aaggtgttcg ccttttatat
      121 tcgattctgg tggtttccaa taatctcatg acatgcgtcc gcccgctatt attgccagcg
      181 acggccggga cttttccatc cctgggctgc taggtcgggt acacgacctc cgttttaccc
      241 gcaacgtaat gctgggaatc tctatcactg atagggagat ctctatcact gatagggaga
      301 gcattgctgc aagggggccg tagaagaagc tctccagcag agtaaaattt cctagggacg
      361 gtaacgggcg gtggaaggag agagaaggga agagacgttt ctggttccat tactccacat
      421 taaagtttta ccccggagaa ttttacgttg ccagcaggtc gttcggagtt gcaacggagc
      481 cagatatttc agagatggct acctgattgg acgaggacac caagacattt ctacaaaaaa
      541 ggatcctgtc aagtgaaagg ggcaatttta agatttcaat cttgatggat ttcattgatc
      601 gataatagtc gcatgtgagg ctgaaatgtg ccgatgaaag aaaaagaaaa accttgcaac
      661 aatttggtcc attgtcatca ctgacctaac gtagttaagg gatgctgata ttctttggga
      721 aaaaaaggaa caccatgatc cacggtggaa gactataatt caggaacgga tgtcaaatgt
      781 ccgtttatgg gaccatttcg atggtaattt gatcggaact actggaaaac tgtgattttt
      841 cagagagtct agtaggccat attggtcggg acacgcctga aactgtctgc aaccccacag
      901 ttcgttaaag tc
//
