LOCUS       sequence-153750-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 4. Promoter GAP4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP155
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    157..175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    178..196
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccatgaattc tgtgttttga tagttgttca attgattgaa atagggacaa ataaattaaa
       61 tttaaagtct ttgggtcagg agaaaccaaa attgggaaag gtgttcgcct tttatattcg
      121 attctggtgg tttccaataa tctcatgaca tgcgtctctc tatcactgat agggagatct
      181 ctatcactga tagggacgcc cgctattatt gccagcgacg gccgggactt ttccatccct
      241 gggctgctag gtcgggtaca cgacctccgt tttacccgca acgtaatgct gggaagagca
      301 ttgctgcaag ggggccgtag aagaagctct ccagcagagt aaaatttcct agggacggta
      361 acgggcggtg gaaggagaga gaagggaaga gacgtttctg gttccattac tccacattaa
      421 agttttaccc cggagaattt tacgttgcca gcaggtcgtt cggagttgca acggagccag
      481 atatttcaga gatggctacc tgattggacg aggacaccaa gacatttcta caaaaaagga
      541 tcctgtcaag tgaaaggggc aattttaaga tttcaatctt gatggatttc attgatcgat
      601 aatagtcgca tgtgaggctg aaatgtgccg atgaaagaaa aagaaaaacc ttgcaacaat
      661 ttggtccatt gtcatcactg acctaacgta gttaagggat gctgatattc tttgggaaaa
      721 aaaggaacac catgatccac ggtggaagac tataattcag gaacggatgt caaatgtccg
      781 tttatgggac catttcgatg gtaatttgat cggaactact ggaaaactgt gatttttcag
      841 agagtctagt aggccatatt ggtcgggaca cgcctgaaac tgtctgcaac cccacagttc
      901 gttaaagtc
//
