LOCUS       sequence-153755-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 DROSHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Cirera-Salinas D, Yu J, Bodak M, Ngondo RP, Herbert KM, Ciaudo C
  TITLE     Noncanonical function of DGCR8 controls mESC exit from pluripotency.
  JOURNAL   J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 
            10.1083/jcb.201606073.
  PUBMED    28100686
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073."
COMMENT     Sequence Label: pX330-sgRNA_Drosha_4
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..135
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        215..290
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        387..672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
       61 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      121 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      181 aggacgaaac accgtcaagc actacgacga ccacgtttta gagctagaaa tagcaagtta
      241 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt
      301 tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc
      361 tgcagacaaa tggctctaga ggtacccgtt acataactta cggtaaatgg cccgcctggc
      421 tgaccgccca acgacccccg cccattgacg tcaatagtaa cgccaatagg gactttccat
      481 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      541 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattgt
      601 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      661 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
      721 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
//
