LOCUS       sequence-153757-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 DROSHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Cirera-Salinas D, Yu J, Bodak M, Ngondo RP, Herbert KM, Ciaudo C
  TITLE     Noncanonical function of DGCR8 controls mESC exit from pluripotency.
  JOURNAL   J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 
            10.1083/jcb.201606073.
  PUBMED    28100686
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073."
COMMENT     Sequence Label: pX330-sgRNA_Drosha_2
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..49)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 actttttctt ttttgcctgg ccggcctttt tcgtggccgc cggccttttg tcgcctccca
       61 gctgagacag gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg
      121 cgtccagcac ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact
      181 tgaaggcggc aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct
      241 gctctctgat gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag
      301 cgtcggccag gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt
      361 agtgcttgtg ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca
      421 gcttctcata gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt
      481 cgtttccctt ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct
      541 cgaacaggga gtacttaggc agcttgatga tcaggtcctt tttcacttct ttgtagccct
      601 tggcttccag aaagtcgatg ggattcttct cgaagctgct tctttccatg atggtgatcc
      661 ccagcagctc tttcacactc ttcagtttct tggacttgcc cttttccact ttggccacca
      721 ccagcacaga ataggccacg gtggggctgt cgaagccgcc gtacttctta gggtcccagt
      781 ccttctttct ggcgatcagc ttatcgctgt tcctcttgg
//
