LOCUS       sequence-153761-        8369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retrograde AAV helper vector, carrying rep and modified capsid gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Tervo DG, Hwang BY, Viswanathan S, Gaj T, Lavzin M, Ritola KD, Lindo
            S, Michael S, Kuleshova E, Ojala D, Huang CC, Gerfen CR, Schiller J,
            Dudman JT, Hantman AW, Looger LL, Schaffer DV, Karpova AY
  TITLE     A Designer AAV Variant Permits Efficient Retrograde Access to 
            Projection Neurons.
  JOURNAL   Neuron. 2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 
            10.1016/j.neuron.2016.09.021.
  PUBMED    27720486
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 
            10.1016/j.neuron.2016.09.021."
COMMENT     Sequence Label: rAAV2-retro helper
FEATURES             Location/Qualifiers
     source          1..8369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      692..1205
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(779..798)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     989..1010
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1687..1706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1806..1828
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1866..1884)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1952..2056
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2057..2125,2126..2917)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2275..2294)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3088..3676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3577..3596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3830..3847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   3919..4021
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     CDS             8124..8135
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gctctagagg tcctgtatta gaggtcacgt gagtgttttg cgacattttg cgacaccatg
       61 tggtcacgct gggtatttaa gcccgagtga gcacgcaggg tctccatttt gaagcgggag
      121 gtttgaacgc gcagccacca cggcggggtt ttacgagatt gtgattaagg tccccagcga
      181 ccttgacgag catctgcccg gcatttctga cagctttgtg aactgggtgg ccgagaagga
      241 atgggagttg ccgccagatt ctgacatgga tctgaatctg attgagcagg cacccctgac
      301 cgtggccgag aagctgcatc gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      361 ccaacagttg cgcagcctga atggcgaatg gcgattccgt tgcaatggct ggcggtaata
      421 ttgttctgga tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg
      481 ttattactaa tcaaagaagt attgcgacaa cggttaattt gcgtgatgga cagactcttt
      541 tactcggtgg cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt
      601 ctaaaatccc tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca
      661 cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
      721 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      781 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      841 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      901 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      961 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1021 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcatattgg
     1081 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     1141 acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc
     1201 ggggtacata tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc
     1261 tccagactct caggcaatga cctgatagcc tttgtagaga cctctcaaaa atagctaccc
     1321 tctccggcat gaatttatca gctagaacgg ttgaatatca tattgatggt gatttgactg
     1381 tctccggcct ttctcacccg tttgaatctt tacctacaca ttactcaggc attgcattta
     1441 aaatatatga gggttctaaa aatttttatc cttgcgttga aataaaggct tctcccgcaa
     1501 aagtattaca gggtcataat gtttttggta caaccgattt agctttatgc tctgaggctt
     1561 tattgcttaa ttttgctaat tctttgcctt gcctgtatga tttattggat gttggaatcg
     1621 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     1681 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     1741 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1801 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1861 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1921 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1981 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2041 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2101 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2161 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     2221 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2281 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2341 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2401 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2461 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2521 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2581 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2641 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2701 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2761 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2821 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2881 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2941 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3001 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3061 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3721 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3781 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3841 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3901 tcattaatgc agaattccca tcatcaataa tataccttat tttggattga agccaatatg
     3961 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
     4021 gtagctctag aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc
     4081 atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg
     4141 gaggtttgaa cgcgcagcca ccacggcggg gttttacgag attgtgatta aggtccccag
     4201 cgaccttgac gagcatctgc ccggcatttc tgacagcttt gtgaactggg tggccgagaa
     4261 ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc aggcacccct
     4321 gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg tgagtaaggc
     4381 cccggaggcc cttttctttg tgcaatttga gaagggagag agctacttcc acatgcacgt
     4441 gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga gtcagattcg
     4501 cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa actggttcgc
     4561 ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg agtgctacat
     4621 ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga ctaatatgga
     4681 acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg cgcagcatct
     4741 gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca attctgatgc
     4801 gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt ggctcgtgga
     4861 caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat acatctcctt
     4921 caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg cgggaaagat
     4981 tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg tggaggacat
     5041 ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc aatatgcggc
     5101 ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca tctggctgtt
     5161 tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca ctgtgccctt
     5221 ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg tcgacaagat
     5281 ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg ccaaagccat
     5341 tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc agatagaccc
     5401 gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg ggaactcaac
     5461 gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac tcacccgccg
     5521 tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt tccggtgggc
     5581 aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg gagccaagaa
     5641 aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg agtcagttgc
     5701 gcagccatcg acgtcagacg cggaagcttc gatcaactac gcagacaggt accaaaacaa
     5761 atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat gcgagagaat
     5821 gaaccagaat tcaaatatct gcttcactca cggacagaaa gactgtttag agtgctttcc
     5881 cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac tgtgctacat
     5941 tcatcatatc atgggaaagg tgccagacgc ttgcactgcc tgcgatctgg tcaatgtggg
     6001 tttggatgac tgcatctttg aacaataaat gatttaaatc aggtatggct gccgatggtt
     6061 atcttccaga ttggctcgag gacactctct ctgaaggaat aagacagtgg tggaagctca
     6121 aacctggccc accaccacca aagcccgcag agcggcataa ggacgacagc aggggtcttg
     6181 tgcttcctgg gtacaagtac ctcggaccct tcaacggact cgacaaggga gagccggtca
     6241 acgaggcaga cgccgcggcc ctcgagcacg acaaagccta cgaccggcag ctcgacagcg
     6301 gagacaaccc gtacctcaag tacaaccacg ccgacgcgga gtttcaggag cgccttaaag
     6361 aagatacgtc ttttgggggc aacctcggac gagcagtctt ccaggcgaaa aagagggttc
     6421 ttgaacctct gggcctggtt gaggaacctg ttaagacggc tccgggaaaa aagaggccgg
     6481 tagagcactc tcctgtggag ccagactcct cctcgggaac cggaaaggcg ggccagcagc
     6541 ctgcaagaaa aagattgaat tttggtcaga ctggagacgc agactcagta cctgaccccc
     6601 agcctctcgg acagccacca gcagccccct ctggtctggg aactaatacg atggctacag
     6661 gcagtggcgc accaatggca gacaataacg agggcgccga cggagtgggt aattcctcgg
     6721 gaaattggca ttgcgattcc acatggatgg gcgacagagt catcaccacc agcacccgaa
     6781 cctgggccct gcccacctac aacaaccacc tctacaaaca aatttccagc caatcaggag
     6841 cctcgaacga caatcactac tttggctaca gcaccccttg ggggtatttt gacttcaaca
     6901 gattccactg ccacttttca ccacgtgact ggcaaagact catcaacaac aactggggat
     6961 tccgacccaa gagactcaac ttcaagctct ttaacattca agtcaaagag gtcacgcaga
     7021 atgacggtac gacgacgatt gccaataacc ttaccagcac ggttcaggtg tttactgact
     7081 cggagtacca gctcccgtac gtcctcggct cggcgcatca aggatgcctc ccgccgttcc
     7141 cagcagacgt cttcatggtg ccacagtatg gatacctcac cctgaacgac gggagtcagg
     7201 cagtaggacg ctcttcattt tactgcctgg agtactttcc ttctcagatg ctgcgtaccg
     7261 gaaacaactt taccttcagc tacacttttg aggacgttcc tttccacagc agctacgctc
     7321 acagccagag tctggaccgt ctcatgaatc ctctcatcga ccagtacctg tattacttga
     7381 gcagaacaaa cactccaagt ggaaccacca cgcagtcaag gcttcagttt tctcaggccg
     7441 gagcgagtga cattcgggac cagtctagga actggcttcc tggaccctgt taccgccagc
     7501 agcgagtatc aaagacatct gcggataaca acaacagtga atactcgtgg actggagcta
     7561 ccaagtacca cctcaatggc agagactctc tggtgaatcc gggcccggcc atggcaagcc
     7621 acaaggacga tgaagaaaag ttttttcctc agagcggggt tctcatcttt gggaagcaag
     7681 gctcagagaa aacaaatgtg gacattgaaa aggtcatgat tacagacgaa gaggaaatca
     7741 ggacaaccaa tcccgtggct acggagcagt atggttctgt atctaccaac ctccagagag
     7801 gcaacctagc agaccaagac tacacaaaaa ctgctaggca agcagctacc gcagatgtca
     7861 acacacaagg cgttcttcca ggcatggtct ggcaggacag agatgtgtac cttcaggggc
     7921 ccatctgggc aaagattcca cacacggacg gacattttca cccctctccc ctcatgggtg
     7981 gattcggact taaacaccct cctccccaga ttctcatcaa gaacaccccg gtacctgcga
     8041 atccttcgac caccttcagt gcggcaaagt ttgcttcctt catcacacag tactccacgg
     8101 gacaggtcag cgtggagatc gagtgggagc tgcagaagga aaacagcaaa cgctggaatc
     8161 ccgaaattca gtacacttcc aactacaaca agtctattaa tgtggacttt actgtggaca
     8221 ctaatggcgt gtattcagag cctcgcccca ttggcaccag atacctgact cgtaatctgt
     8281 aattgcggcc gcttgttaat caataaaccg tttaattcgt ttcagttgaa ctttggtctc
     8341 tgcgtatttc tttcttatct agtttccat
//
