LOCUS       sequence-153762-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: HLCS_pCSdest
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgga agatagactc cacatggata atggactggt accccaaaag
      181 attgtgtcgg tgcacttgca ggactccact ctgaaggaag ttaaggatca ggtctcaaac
      241 aagcaagccc agatcctaga gccgaagcct gaaccttctc ttgagattaa gcctgagcag
      301 gacggtatgg agcatgttgg cagagatgac ccaaaggctc ttggtgaaga acccaaacaa
      361 aggagaggca gtgcctctgg gagtgagcct gctggggaca gtgacagggg agggggcccc
      421 gttgagcatt atcacctcca tctgtctagt tgccacgagt gtctggaact tgagaacagc
      481 accattgagt cagtcaagtt tgcgtctgcc gagaacattc cagaccttcc ctacgattat
      541 agcagcagtt tggagagtgt tgctgatgag acctcccccg aaagagaagg gaggagagtc
      601 aacctcacgg gaaaggcacc caacatcctc ctctatgtgg gctccgactc ccaggaagcc
      661 ctcggccggt tccacgaggt ccggtctgtg ctggccgact gtgtggacat tgacagttat
      721 attctctacc acctgctgga ggacagtgct ctcagagacc cgtggacgga caactgtctg
      781 ctgttggtca ttgctaccag g
//
