LOCUS       sequence-153768-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: NCAM2_pCSdest
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgag cctcctcctc tccttctacc tgctggggtt gcttgtcagt
      181 agcgggcaag ctcttcttca agtgacaatt tcacttagca aagtagagct tagtgttgga
      241 gaatctaaat tcttcacatg tacagcgatt ggtgaacctg aaagtataga ttggtataat
      301 cctcaaggag agaagataat ttcaacacag agggtagtag tgcaaaagga aggtgttagg
      361 tcacggttaa ccatctacaa tgcaaatata gaagatgcag ggatatatcg ttgtcaagca
      421 acagatgcca aaggacaaac acaagaagct acagtagttt tggaaattta ccaaaaactc
      481 actttcagag aagtggtatc tccacaagaa ttcaaacaag gagaagatgc agaagtggtt
      541 tgccgagtta gcagttcacc tgcacctgct gtcagctggt tgtatcataa tgaggaagtc
      601 accactattt ccgacaatcg gttcgctatg ttagcaaaca ataacctgca gattctcaac
      661 atcaataaaa gtgatgaagg tatatacaga tgtgaaggaa gagtggaggc caggggagaa
      721 attgacttcc gtgatatcat tgttattgtt aatgtgccgc cagcaatctc aatgcctcag
      781 aa
//
