LOCUS       sequence-153770-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP p6 with K27R mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Gurer C, Berthoux L, Luban J
  TITLE     Covalent modification of human immunodeficiency virus type 1 p6 by 
            SUMO-1.
  JOURNAL   J Virol. 2005 Jan;79(2):910-7.
  PUBMED    15613319
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2005-01"; volume: "79(2)"; pages: "910-7"
COMMENT     Sequence Label: pcDNA 3.1(+) eGFP p6 K27R
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      277..286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(350..371)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     699..718
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
       61 accacactgg actagtggat ccgagctcgg taccgggccc cccctcgagg tcgagttatt
      121 gtgacgaggg gtcgttgcca aagagtgatc tgagggaagt taaaggatac agttccttgt
      181 ctataggttc ttgtctttga gggggagttg ttgtctctac cccagacctg aagctctctt
      241 ctggtggggc tgttggctct ggtctgctct gaagtcccat ggcggccgct ctcgagttaa
      301 cagatctctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      361 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
      421 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      481 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcgccttga
      541 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
      601 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
      661 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
      721 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
      781 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
      841 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
      901 tgccgtaggt ggcatcgcc
//
