LOCUS       sequence-153774-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR10_pCSdest
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(434..458)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    502..636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(526..545)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     580..599
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(749..769)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(749..767)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(788..804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(788..804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(801..823)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    812..828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gctccgcggc cgcccccttc accatgcaga ttgtgcaggg gttccctgcg
      181 gatgcacccc tgtgtgccct gatgtggacc tgctccttcc tgcttcctgg gctccagaca
      241 gaaacaccgt acccctgcac cagcctctgc ctcagctctt cacagtcagc acaccctccg
      301 cttcctgtca gggtgttctc tgctgagtct ggttatggga tccctttctg tgcagagcca
      361 tgctcccgtg tcactgtgtg tcaccttcaa gctgtccccg tgtgtatgcc tgtgtgaaag
      421 ggtgggcgcg ccgacccagc tttcttgtac aaagtggtga tcctctcgag cctctagaac
      481 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
      541 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      601 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
      661 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg
      721 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
      781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      841 tacgagccgg
//
