LOCUS       sequence-153775-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR10_pCSdest
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(363..387)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(441..459)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(442..459)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gtcggcgcgc ccaccctttc acacaggcat acacacgggg acagcttgaa ggtgacacac
      121 agtgacacgg gagcatggct ctgcacagaa agggatccca taaccagact cagcagagaa
      181 caccctgaca ggaagcggag ggtgtgctga ctgtgaagag ctgaggcaga ggctggtgca
      241 ggggtacggt gtttctgtct ggagcccagg aagcaggaag gagcaggtcc acatcagggc
      301 acacaggggt gcatccgcag ggaacccctg cacaatctgc atggtgaagg gggcggccgc
      361 ggagcctgct tttttgtaca aacttgtgat ccttgaattc gaatcgatgg gatcctgcaa
      421 aaagaacaag tagcttgtat tctatagtgt cacctaaatc aagcttgctc cgacgtcccc
      481 aggcagaatg gcggttccct aaacgagcat tgcttatata gacctcccat taggcacgcc
      541 taccgcccat ttacgtcaat ggaacgccca tttgcgtcat tgcccctccc cattgacgtc
      601 aatggggatg tacttggcag ccatcgcggg ccatttaccg ccattgacgt caatgggagt
      661 actgccaatg taccctggcg tacttccaat agtaatgtac ttgccaagtt actattaata
      721 gatattgatg tactgccaag tgggccattt accgtcattg acgtcaatag gg
//
