LOCUS       sequence-153785-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: ETS2_pCSdest
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgaa tgatttcgga atcaagaata tggaccaggt agcccctgtg
      181 gctaacagtt acagagggac actcaagcgc cagccagcct ttgacacctt tgatgggtcc
      241 ctgtttgctg tttttccttc tctaaatgaa gagcaaacac tgcaagaagt gccaacaggc
      301 ttggattcca tttctcatga ctccgccaac tgtgaattgc ctttgttaac cccgtgcagc
      361 aaggctgtga tgagtcaagc cttaaaagct accttcagtg gcttcaaaaa ggaacagcgg
      421 cgcctgggca ttccaaagaa cccctggctg tggagtgagc aacaggtatg ccagtggctt
      481 ctctgggcca ccaatgagtt cagtctggtg aacgtgaatc tgcagaggtt cggcatgaat
      541 ggccagatgc tgtgtaacct tggcaaggaa cgctttctgg agctggcacc tgactttgtg
      601 ggtgacattc tctgggaaca tctggagcaa atgatcaaag aaaaccaaga aaagacagaa
      661 gatcaatatg aagaaaattc acacctcacc tccgttcctc attggattaa cagcaataca
      721 ttaggttttg gcacagagca ggcgccctat ggaatgcaga cacagaatta ccccaaaggc
      781 ggcctcctgg acagcatgtg tccggcctcc acacccagcg tactcagctc tgagcag
//
