LOCUS       sequence-153790-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PFKL_pCSdest
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatggc cgcggtggac ctggagaagc tgcgggcgtc gggcgcgggc
      181 aaggccatcg gcgtcctgac cagcggcggc gacgcgcaag gcatgaacgc tgctgtccgg
      241 gctgtgacgc gcatgggcat ttatgtgggt gccaaagtct tcctcatcta cgagggctat
      301 gagggcctcg tggagggagg tgagaacatc aagcaggcca actggctgag cgtctccaac
      361 atcatccagc tgggcggcac tatcattggc agcgctcgct gcaaggcctt taccaccagg
      421 gaggggcgcc gggcagcggc ctacaacctg gtccagcacg gcatcaccaa cctgtgcgtc
      481 atcggcgggg atggcagcct tacaggtgcc aacatcttcc gcagcgagtg gggcagcctg
      541 ctggaggagc tggtggcgga aggtaagatc tcagagacta cagcccggac ctactcgcac
      601 ctgaacatcg cgggcctagt gggctccatc gataacgact tctgcggcac cgacatgacc
      661 atcggcacgg actcggccct ccaccgcatc atggaggtca tcgatgccat caccaccact
      721 gcccagagcc accagaggac cttcgtgctg gaagtgatgg gccggcactg cgg
//
