LOCUS       sequence-153794-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for stable expression of osTIR1-9Myc in mammalian 
            cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Lambrus BG, Moyer TC, Holland AJ
  TITLE     Applying the auxin-inducible degradation system for rapid protein 
            depletion in mammalian cells.
  JOURNAL   Methods Cell Biol. 2018;144:107-135. doi: 
            10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5.
  PUBMED    29804665
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Cell Biol."; date: "2018"; volume: "144:107-135. doi"; pages: " 
            10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5"
COMMENT     Sequence Label: pBabe Blast osTIR1-9Myc
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..473
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
       61 ttgcaaaaag cttcatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
      121 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
      181 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
      241 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
      301 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
      361 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
      421 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagatcgat
      481 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
      541 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
      601 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
      661 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
      721 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
      781 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
      841 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
      901 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
      961 cactcggg
//
