LOCUS       sequence-153795-       12382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Dajee M, Tarutani M, Deng H, Cai T, Khavari PA
  TITLE     Epidermal Ras blockade demonstrates spatially localized Ras 
            promotion of proliferation and inhibition of differentiation
  JOURNAL   Oncogene. 2002 Feb 28;21(10):1527-38
  PUBMED    11896581
REFERENCE   2  (bases 1 to 12382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2002-02-28"; volume: "21(10)"; pages: "1527-3"
COMMENT     Sequence Label: LZRS ERTm RasV12
FEATURES             Location/Qualifiers
     source          1..12382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1045..1077,1078..1080,1081..1614)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     LTR             1835..2304
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2434..2451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2605..3193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2685..2704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3316..3450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3340..3359)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3394..3413
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3680..4279)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3763..3783)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4260..4279
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4362..4861)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4410..4429)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4803..4826
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      6893..8682
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(9176..9967,9968..10036))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9799..9818
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10037..10141)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             10678..11147
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    11212..11569
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             11634..12050
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     11980..12002
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     12018..12034
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(12060..12382,1..52)
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     join(12372..12382,1..6)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 cgccgcccac gtgaaggctg ccgaccccgg gggtggacca tcctctagac tgccggatcc
       61 accatgcgaa atgaaatggg tgcttcagga gacatgaggg ctgccaacct ttggccaagc
      121 cctcttgtga ttaagcacac taagaagaat agccctgcct tgtccttgac agctgaccag
      181 atggtcagtg ccttgttgga tgctgaaccg cccatgatct attctgaata tgatccttct
      241 agacccttca gtgaagcctc aatgatgggc ttattgacca acctagcaga tagggagctg
      301 gttcatatga tcaactgggc aaagagagtg ccaggctttg gggacttgaa tctccatgat
      361 caggtccacc ttctcgagtg tgcctggctg gagattctga tgattggtct cgtctggcgc
      421 tccatggaac acccggggaa gctcctgttt gctcctaact tgctcctgga caggaatcaa
      481 ggtaaatgtg tggaaggcat ggtggagatc tttgacatgt tgcttgctac gtcaagtcgg
      541 ttccgcatga tgaacctgca gggtgaagag tttgtgtgcc tcaaatccat cattttgctt
      601 aattccggag tgtacacgtt tctgtccagc accttgaagt ctctggaaga gaaggaccac
      661 atccaccgtg tcctggacaa gatcacagac actttgatcc acctgatggc caaagctggc
      721 ctgactctgc agcagcagca tcgccgccta gctcagctcc ttctcattct ttcccatatc
      781 cggcatatga gtaacaaacg catggagcat ctctacaaca tgaaatgcaa gaacgtggta
      841 cccctctatg acctgctcct ggagatgttg gatgcccacc gccttcatgc cccagccagt
      901 cgcatgggag tgcccccaga ggagcccagc cagacccagc tggccaccac cagctccact
      961 tcagcacatt ccttacaaac ctactacata cccccggaag cagagggctt ccccaacgat
     1021 atcccacgtg gaggcctgaa ttccatgacg gaatataagc tggtggtggt gggcgccgtc
     1081 ggtgtgggca agagtgcgct gaccatccag ctgatccaga accattttgt ggacgaatac
     1141 gaccccacta tagaggattc ctaccggaag caggtggtca ttgatgggga gacgtgcctg
     1201 ttggacatcc tggataccgc cggccaggag gagtacagcg ccatgcggga ccagtacatg
     1261 cgcaccgggg agggcttcct gtgtgtgttt gccatcaaca acaccaagtc ttttgaggac
     1321 atccaccagt acagggagca gatcaaacgg gtgaaggact cggatgacgt gcccatggtg
     1381 ctggtgggga acaagtgtga cctggctgca cgcactgtgg aatctcggca ggctcaggac
     1441 ctcgcccgaa gctacggcat cccctacatc gagacctcgg ccaagacccg gcagggagtg
     1501 gaggatgcct tctacacgtt ggtgcgtgag atccggcagc acaagctgcg gaagctgaac
     1561 cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc ctgacgcaat
     1621 tcgatatcaa gcttgaattc ccgcgggcgg ccgccagcac agtggtcgac gataaaataa
     1681 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     1741 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
     1801 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     1861 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     1921 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     1981 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     2041 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     2101 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     2161 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     2221 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     2281 ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt
     2341 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag
     2401 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3301 tttgcggccg aattagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     3361 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3421 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3481 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     3541 atggctgatt atgatcctct agagtcggtg ggcctcgggg gcgggtgcgg ggtcggcggg
     3601 gccgccccgg gtggcttcgg tcggagccat ggggtcgtgc gctcctttcg gtcgggcgct
     3661 gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc
     3721 cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt
     3781 tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc
     3841 cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag acgccgacgg
     3901 tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt
     3961 gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca
     4021 ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg
     4081 cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg
     4141 tgacccgctc gatgtggcgg tccgggtcga cggtgtggcg cgtggcgggg tagtcggcga
     4201 acgcggcggc gagggtgcgt acggcccggg ggacgtcgtc gcgggtggcg aggcgcaccg
     4261 tgggcttgta ctcggtcatg gaaggtcgtc tccttgtgag gggtcagggg cgtgggtcag
     4321 gggatggtgg cggcaccggt cgtggcggcc gacctgcagg tcgaaaggcc cggagatgag
     4381 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
     4441 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
     4501 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
     4561 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
     4621 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
     4681 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
     4741 cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg
     4801 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
     4861 cggtagaatt aattctcatg tttgacagct tatcatcgat aagctgatcc tcacaggccg
     4921 cacccagctt ttcttccgtt gccccagtag catctctgtc tggtgacctt gaagaggaag
     4981 aggaggggtc ccgagaatcc ccatccctac cgtccagcaa aaagggggac gaggaatttg
     5041 aggcctggct tgaggctcag gacgcaaatc ttgaggatgt tcagcgggag ttttccgggc
     5101 tgcgagtaat tggtgatgag gacgaggatg gttcggagga tggggaattt tcagacctgg
     5161 atctgtctga cagcgaccat gaaggggatg agggtggggg ggctgttgga gggggcagga
     5221 gtctgcactc cctgtattca ctgagcgtcg tctaataaag atgtctattg atctctttta
     5281 gtgtgaatca tgtctgacga ggggccaggt acaggacctg gaaatggcct aggagagaag
     5341 ggagacacat ctggaccaga aggctccggc ggcagtggac ctcaaagaag agggggtgat
     5401 aaccatggac gaggacgggg aagaggacga ggacgaggag gcggaagacc aggagccccg
     5461 ggcggctcag gatcagggcc aagacataga gatggtgtcc ggagacccca aaaacgtcca
     5521 agttgcattg gctgcaaagg gacccacggt ggaacaggag caggagcagg agcgggaggg
     5581 gcaggagcag gaggggcagg agcaggagga ggggcaggag caggaggggc aggagcagga
     5641 ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc gaggaggtag tggaggccgg
     5701 ggtcgaggag gtagtggagg ccgccggggt agaggacgtg aaagagccag ggggggaagt
     5761 cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa agaggcccag gagtcccagt
     5821 agtcagtcat catcatccgg gtctccaccg cgcaggcccc ctccaggtag aaggccattt
     5881 ttccaccctg taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt
     5941 gagcctgacg tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc
     6001 ccaagcactg gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt
     6061 ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta
     6121 agagctctcc tggctaggag tcacgtagaa aggactaccg acgaaggaac ttgggtcgcc
     6181 ggtgtgttcg tatatggagg tagtaagacc tccctttaca acctaaggcg aggaactgcc
     6241 cttgctattc cacaatgtcg tcttacacca ttgagtcgtc tcccctttgg aatggcccct
     6301 ggacccggcc cacaacctgg cccgctaagg gagtccattg tctgttattt catggtcttt
     6361 ttacaaactc atatatttgc tgaggttttg aaggatgcga ttaaggacct tgttatgaca
     6421 aagcccgctc ctacctgcaa tatcagggtg actgtgtgca gctttgacga tggagtagat
     6481 ttgcctccct ggtttccacc tatggtggaa ggggctgccg cggagggtga tgacggagat
     6541 gacggagatg aaggaggtga tggagatgag ggtgaggaag ggcaggagtg atgtaacttg
     6601 ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc cccgcactaa agaataaatc
     6661 cccagtagac atcatgcgtg ctgttggtgt atttctggcc atctgtcttg tcaccatttt
     6721 cgtcctccca acatggggca attgggcata cccatgttgt cacgtcactc agctccgcgc
     6781 tcaacacctt ctcgcgttgg aaaacattag cgacatttac ctggtgagca atcagacatg
     6841 cgacggcttt agcctggcct ccttaaattc acctaagaat gggagcaacc agcaggaaaa
     6901 ggacaagcag cgaaaattca cgcccccttg ggaggtggcg gcatatgcaa aggatagcac
     6961 tcccactcta ctactgggta tcatatgctg actgtatatg catgaggata gcatatgcta
     7021 cccggataca gattaggata gcatatacta cccagatata gattaggata gcatatgcta
     7081 cccagatata gattaggata gcctatgcta cccagatata aattaggata gcatatacta
     7141 cccagatata gattaggata gcatatgcta cccagatata gattaggata gcctatgcta
     7201 cccagatata gattaggata gcatatgcta cccagatata gattaggata gcatatgcta
     7261 tccagatatt tgggtagtat atgctaccca gatataaatt aggatagcat atactaccct
     7321 aatctctatt aggatagcat atgctacccg gatacagatt aggatagcat atactaccca
     7381 gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca
     7441 gatataaatt aggatagcat atactaccca gatatagatt aggatagcat atgctaccca
     7501 gatatagatt aggatagcct atgctaccca gatatagatt aggatagcat atgctatcca
     7561 gatatttggg tagtatatgc tacccatggc aacattagcc caccgtgctc tcagcgacct
     7621 cgtgaatatg aggaccaaca accctgtgct tggcgctcag gcgcaagtgt gtgtaatttg
     7681 tcctccagat cgcagcaatc gcgcccctat cttggcccgc ccacctactt atgcaggtat
     7741 tccccggggt gccattagtg gttttgtggg caagtggttt gaccgcagtg gttagcgggg
     7801 ttacaatcag ccaagttatt acacccttat tttacagtcc aaaaccgcag ggcggcgtgt
     7861 gggggctgac gcgtgccccc actccacaat ttcaaaaaaa agagtggcca cttgtctttg
     7921 tttatgggcc ccattggcgt ggagccccgt ttaattttcg ggggtgttag agacaaccag
     7981 tggagtccgc tgctgtcggc gtccactctc tttccccttg ttacaaatag agtgtaacaa
     8041 catggttcac ctgtcttggt ccctgcctgg gacacatctt aataacccca gtatcatatt
     8101 gcactaggat tatgtgttgc ccatagccat aaattcgtgt gagatggaca tccagtcttt
     8161 acggcttgtc cccaccccat ggatttctat tgttaaagat attcagaatg tttcattcct
     8221 acactagtat ttattgccca aggggtttgt gagggttata ttggtgtcat agcacaatgc
     8281 caccactgaa ccccccgtcc aaattttatt ctgggggcgt cacctgaaac cttgttttcg
     8341 agcacctcac atacacctta ctgttcacaa ctcagcagtt attctattag ctaaacgaag
     8401 gagaatgaag aagcaggcga agattcagga gagttcactg cccgctcctt gatcttcagc
     8461 cactgccctt gtgactaaaa tggttcacta ccctcgtgga atcctgaccc catgtaaata
     8521 aaaccgtgac agctcatggg gtgggagata tcgctgttcc ttaggaccct tttactaacc
     8581 ctaattcgat agcatatgct tcccgttggg taacatatgc tattgaatta gggttagtct
     8641 ggatagtata tactactacc cgggaagcat atgctacccg tttagggtta acaagggggc
     8701 cttataaaca ctattgctaa tgccctcttg agggtccgct tatcggtagc tacacaggcc
     8761 cctctgattg acgttggtgt agcctcccgt agtcttcctg ggcccctggg aggtacatgt
     8821 cccccagcat tggtgtaaga gcttcagcca agagttacac ataaaggcaa tgttgtgttg
     8881 cagtccacag actgcaaagt ctgctccagg atgaaagcca ctcagtgttg gcaaatgtgc
     8941 acatccattt ataaggatgt caactacagt cagagaaccc ctttgtgttt ggtccccccc
     9001 cgtgtcacat gtggaacagg gcccagttgg caagttgtac caaccaactg aagggattac
     9061 atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc aggtttagtt cgtccggggc
     9121 ggggatctgg ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10141 gcacatttcc ccgaaaagtg ccaccagctt tgctcttagg agtttcctaa tacatcccaa
    10201 actcaaatat ataaagcatt tgacttgttc tatgccctag ggggcggggg gaagctaagc
    10261 cagctttttt taacatttaa aatgttaatt ccattttaaa tgcacagatg tttttatttc
    10321 ataagggttt caatgtgcat gaatgctgca atattcctgt taccaaagct agtataaata
    10381 aaaatagata aacgtggaaa ttacttagag tttctgtcat taacgtttcc ttcctcagtt
    10441 gacaacataa atgcgctgct gagaagccag tttgcatctg tcaggatcaa tttcccatta
    10501 tgccagtcat attaattact agtcaattag ttgattttta tttttgacat atacatgtga
    10561 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
    10621 aaaatacata actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg
    10681 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
    10741 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
    10801 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
    10861 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
    10921 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
    10981 agagcccaca acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac
    11041 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
    11101 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
    11161 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
    11221 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg
    11281 tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
    11341 cggaacaccc ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg
    11401 cccgacctga gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg
    11461 agggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
    11521 ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc
    11581 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga
    11641 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
    11701 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
    11761 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
    11821 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
    11881 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
    11941 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
    12001 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc
    12061 catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga
    12121 caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc
    12181 acgaagtctg gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac
    12241 ctcaccctta ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag
    12301 aacctcgctg gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag
    12361 acggcatcgc agcttggata ca
//
