LOCUS       sequence-153798-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Sen GL, Webster DE, Barragan DI, Chang HY, Khavari PA
  TITLE     Control of differentiation in a self-renewing mammalian tissue by 
            the histone demethylase JMJD3.
  JOURNAL   Genes Dev. 2008 Jul 15. 22(14):1865-70.
  PUBMED    18628393
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Jul 15. 22(14):1865-70."
COMMENT     Sequence Label: MSCV JMJD3 mutant
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             86..112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    128..152
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(573..584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tccagccctc actccttctc taggcgccgg aattagatct gccaccatgg actacaagga
       61 tgacgatgac aagctcgatg gaggataccc atacgatgtt ccagattacg ctgctcgagg
      121 ttatcaaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccggatccat
      181 gcatcgggca gtggatcctc caggggcccg cgctgcacgg gaagcctttg cccttggggg
      241 cctgagctgt gctggggcct ggagctcctg cccgcctcat ccccctcctc gtagcgcatg
      301 gctgcctgga ggcagatgct cagccagcat tgggcagccc ccgcttcctg ctcccctacc
      361 cccttcacat ggcagtagtt ctgggcaccc cagcaaacca tattatgctc caggggcgcc
      421 cactccaaga cccctccatg ggaagctgga atccctgcat ggctgtgtgc aggcattgct
      481 ccgggagcca gcccagccag ggctttggga acagcttggg caactgtacg agtcagagca
      541 cgatagtgag gaggccacac gctgctacca cagcgccctt cgatacggag gaagcttcgc
      601 tgagctgggg ccccgcattg gccgactgca gcaggcccag ctctggaact ttcatactgg
      661 ctcctgccag caccgagcca aggtcctgcc cccactggag caagtgtgga acttgctaca
      721 ccttgagcac aaacggaact atggagccaa gcggggaggt cccccggtga agcgagctgc
      781 tgaaccccca gtggtgcagc ctgtgcctcc tgcagcactc tcaggcccct cagg
//
