LOCUS       sequence-153804-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for N-terminal EGFP-AID tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     pBabe Neo OsTIR1-9Myc
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA5-FRT-TO-EGFP-AID
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..778)
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     primer_bind     complement(836..857)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctctagact cgagcggccg ccactgtgct ggatatctgc agaattccac cacactggac
       61 tagtggatcc agatccgcca gcagcggcgg ctccggaagc tctgctcttg cacttctcca
      121 tcgccctcgg agcgagacca atggcatccg atcctttcat gagacgtaaa cgcttgcatg
      181 tatcgacgaa cattggccaa ggaacgtcgc cgacgagcat ccaatcaccg tctttgtctt
      241 cataagaggg aacatagtcc cagctattca ccaaatccat caatttcctc tcattcatga
      301 agtctatcat tccttcttct cctccatgtt tgcccatggt aaaagagctg aacatgttgg
      361 acaaagcatt agaaagctca tcgtagcttt tatacatcct caaatcgatt ttcctcaagt
      421 acggtgctcc gtccattgat accttcacga acgccgccgc ctccgggcca ccgcttgatt
      481 tttggcagga aaccatcacg ttcttccggt atgatctcac cggtggccat cccacaactt
      541 gtgccttggc cggaggtttg gctggatctt taggacaagc actcttctcc ttggaatcaa
      601 aagtcacgac gtcatgagtc gtagatcctt ccttgtttgc aggctcatta ttcagattta
      661 gcttcagatc aaccgtctct gagaaccctc tcttgtttcc ggttaccgga gccactgtat
      721 ctccaccggg aagaccaaga cacagctcag tctccctcag attcagctcg acactgccag
      781 atccgccgct agccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      841 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      901 tgctcaggta gtggttgtcg ggcagcagca cgg
//
