LOCUS       sequence-153806-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single fluorescent protein biosensor for trehalose.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Nadler DC, Morgan SA, Flamholz A, Kortright KE, Savage DF
  TITLE     Rapid construction of metabolite biosensors using domain-insertion 
            profiling.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
  PUBMED    27470466
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12266. doi"; pages: " 
            10.1038/ncomms12266"
COMMENT     Sequence Label: pTKEI-Tre-C04
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     634..655
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(761..782)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatggtgatg gtgaccgctt gaatactgct ttaccagttc ttcggcttct ttttgagctt
       61 tatccagtgc ttcctgcggg gagattttgc ctgcaagggc ggagttaacg tatttctgga
      121 tgatttcact cagttgcggg taatacggta cgattgggcg tgggaccgcg ttttcaaaca
      181 ccgcacgcag ctctttcaga tgggggcttt tggacaccac cgccggatcg tcgtatacgt
      241 ctacgcgacc gcggctgcgg ttgaagttat attcaagctt atggcccagg atgttgccgt
      301 cctccttgaa gtcgatgccc ttcagctcga tgcggttaac cagagtgtcg ccctcgaact
      361 taacctcagc gcgtgtctta tagttgccgt cgtccttgaa gaagatggtg cgctcctgaa
      421 tgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      481 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
      541 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
      601 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
      661 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat gctccctccg gtaccgccct
      721 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      781 tgtgatcgcg cttctcgttg gggtctttgc tcagtttgga ctgcacgctc aggtagtgg
//
